Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China

doi:10.1016/j.jes.2018.05.029

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 藻类生物学及应用研究中心

	Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China
	Gu, Yilu 1,2; Gao, Xiaofeng 2; Jiang, Yongguang 3; Peng, Xin 1; Li, Renhui 1
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2019-02-01
卷号	76 页码:359-367
关键词	Microcystis panniformis Blooms ITS of 16S-23S rRNA mcyE Microcystin Taihu
ISSN号	1001-0742
DOI	10.1016/j.jes.2018.05.029
通讯作者	Li, Renhui(reli@ihb.ac.cn)
英文摘要	Microcystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morphological examination on colonial transition and genetic examination are needed. Three M. panniformis strains isolated from a water bloom sample in Lake Taihu were characterized by molecular analysis and toxin quantification. Phylogenetic analysis based on both 16S rRNA gene and internal transcribed spacer (ITS) between 16S and 23S rRNA genes were performed and compared to facilitate easy identification of the species. Relatively high similarities (98%-99%) were shown in 16S rDNA sequences between the strains of M. panniformis and those of other Microcystis species, whereas the similarities for ITS sequences were 88%-95%. In the phylogenetic tree based on the 16S rDNA sequences, the M. panniformis and M. aeruginosa strains were intermixed together with no clear division, whereas all of the M. panniformis strains were clustered together in a single Glade based on the ITS sequences based phylogenyetic tree. The mcyE gene was detected in all three strains, and microcystin was determined by high-performance liquid chromatography. The molecular detection and toxin production of M. panniformis strains are of great significance for the environmental risk assessment of Microcystis blooms. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
资助项目	National Water Science and Technology Project[2017ZX07203-003] ; National Basic Research Program of China[2017YFA0605201] ; National Natural Science Foundation of China[412111020]
WOS关键词	WESENBERGII KOMAREK KOMAREK ; GENETIC DIVERSITY ; WATER-BLOOM ; PHYLOGENETIC-RELATIONSHIPS ; CYANOBACTERIA ; POPULATIONS ; STRAINS ; CULTURE ; 16S ; MORPHOLOGY
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000463373300034
资助机构	National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27787]
专题	水生生物研究所_藻类生物学及应用研究中心
通讯作者	Li, Renhui
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 2.Univ Tokyo, Dept Urban Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan 3.China Univ Geosci, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Gu, Yilu,Gao, Xiaofeng,Jiang, Yongguang,et al. Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2019,76:359-367.
APA	Gu, Yilu,Gao, Xiaofeng,Jiang, Yongguang,Peng, Xin,&Li, Renhui.(2019).Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,76,359-367.
MLA	Gu, Yilu,et al."Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 76(2019):359-367.