In vivo tumor imaging by a gamma-glutamyl transpeptidase-activatable near-infrared fluorescent probe

doi:10.1007/s00216-018-1181-9

	In vivo tumor imaging by a gamma-glutamyl transpeptidase-activatable near-infrared fluorescent probe
	Li, Lihong 1,2; Shi, Wen 1; Wu, Xiaofeng 1; Li, Xiaohua 1; Ma, Huimin 1
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2018-10-01
卷号	410 期号:26 页码:6771-6777
关键词	Gamma-glutamyl Transpeptidase Near-infrared Fluorescent Probe Imaging In Vivo
ISSN号	1618-2642
DOI	10.1007/s00216-018-1181-9
英文摘要	gamma-Glutamyl transpeptidase (GGT), overexpressed in various cancer cells, has been perceived as a latent tumor biomarker. Thus, developing near-infrared (NIR) fluorescent GGT probes is highly desired for in vivo tumor imaging and studies. To our knowledge, however, such a GGT probe is still rare. Herein, we construct a new GGT-activatable NIR fluorescent probe HCAGlu by incorporating -glutamyl group as a recognition unit directly into a NIR hemicyanine fluorophore. HCAGlu exhibits a highly sensitive and selective NIR fluorescence off-on response to GGT. The probe has been applied to cell and histological section imaging, which demonstrates the ability of HCAGlu in distinguishing different GGT-expression levels in situ in biological samples. Notably, in vivo fluorescence imaging in tumor-bearing mice has been performed, verifying that probe HCAGlu can rapidly produce a distinct fluorescence signal in the tumor site via both intravenous and intratumoral injections. The simplicity and excellent performance of HCAGlu make it of high potential in studying the physiological function of GGT in vivo.
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000444236400008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/41884]
专题	化学研究所_活体分析化学实验室
通讯作者	Shi, Wen; Ma, Huimin
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Shanxi Med Univ, Dept Chem, Taiyuan 030001, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Lihong,Shi, Wen,Wu, Xiaofeng,et al. In vivo tumor imaging by a gamma-glutamyl transpeptidase-activatable near-infrared fluorescent probe[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(26):6771-6777.
APA	Li, Lihong,Shi, Wen,Wu, Xiaofeng,Li, Xiaohua,&Ma, Huimin.(2018).In vivo tumor imaging by a gamma-glutamyl transpeptidase-activatable near-infrared fluorescent probe.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(26),6771-6777.
MLA	Li, Lihong,et al."In vivo tumor imaging by a gamma-glutamyl transpeptidase-activatable near-infrared fluorescent probe".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.26(2018):6771-6777.