Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase

doi:10.1365/s10337-004-0276-y

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	Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase
	Yang, GL ; Yin, JF ; Li, ZW ; Liu, HY ; Cai, LP ; Wang, DX ; Chen, Y
刊名	CHROMATOGRAPHIA
	2004-06-01
卷号	59 期号:11-12 页码:705-708
关键词	Column Liquid Chromatography Molecularly Imprinted Stationary Phase Chiral Separation Nateglinide
ISSN号	0009-5893
DOI	10.1365/s10337-004-0276-y
英文摘要	A new chiral stationary phase for nateglinide (N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine) based on a molecularly imprinted polymer has been prepared by non-covalent imprinting. For chromatographic analysis the effects on the separation of mobile phase composition, flow rate, and temperature were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, acetonitrile; flow rate, 0.5 mL min(-1); temperature, 25 degreesC. It was shown that the noteglinide-imprinted polymer was capable of recognizing the enantiomeric difference between nateglinide and its L enantiomer, whereas the non-imprinted polymer had no such ability. Scatchard analysis was used to investigate the binding characteristics of the noteglinide-imprinted stationary phase; this indicated that two classes of binding site were present in the imprinted polymer. The equilibrium dissociation constant (K-D) and the apparent maximum number (Q(max)) of high- and low-affinity binding sites were 3.7 x 10(-4) mol L-1 and 11.38 mumol g(-1), and 1.81 x 10(-3) mol L-1 and 27.73 mumol g(-1), respectively.
语种	英语
出版者	VIEWEG
WOS记录号	WOS:000224100300008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/82411]
专题	中国科学院化学研究所
通讯作者	Yang, GL
作者单位	1.Hebei Univ Baoding, Coll Pharm, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yang, GL,Yin, JF,Li, ZW,et al. Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase[J]. CHROMATOGRAPHIA,2004,59(11-12):705-708.
APA	Yang, GL.,Yin, JF.,Li, ZW.,Liu, HY.,Cai, LP.,...&Chen, Y.(2004).Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase.CHROMATOGRAPHIA,59(11-12),705-708.
MLA	Yang, GL,et al."Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase".CHROMATOGRAPHIA 59.11-12(2004):705-708.