Distance-dependent long-range electron transfer in protein: a case study of photosynthetic bacterial light-harvesting antenna complex LH2 assembled on TiO2 nanoparticle by femto-second time-resolved spectroscopy

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	Distance-dependent long-range electron transfer in protein: a case study of photosynthetic bacterial light-harvesting antenna complex LH2 assembled on TiO2 nanoparticle by femto-second time-resolved spectroscopy
	Weng, YX ; Zhang, L ; Yang, J ; Quan, DH ; Wang, L ; Yang, GZ ; Ritsuko, F ; Yasushi, K ; Zhang, JP ; Feng, J
刊名	ACTA BOTANICA SINICA
	2003-04-01
卷号	45 期号:4 页码:488-493
关键词	Tio2 Nanoparticle Lh2 Time-resolved Spectroscopy Charge Transfer Energy Transfer Protein
ISSN号	0577-7496
英文摘要	The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna complex assembled onto TiO2 nanoparticle with an average size of 8 nm in diameter. Crystal structure shows that photosynthetic bacterial antenna complex LH2 has a ring-like structure composed by alpha- and beta-apoprotein helices. The alpha- and beta-transmembrance helices construct two concentric cylinders with pigments bacteriochlorophyll a (Bchl a) and carotenoid (Car) buried inside the protein. We attempt to insert TiO2 nanoparticle into the cavity of the inner cylindrical hollow of LH2 to investigate the nature of the electron transfer between the excited-state Bchl a and the TiO2 nanoparticle. A significant decrease in the ground state bleaching recovery time constant for Bchl a at 850 run (B850) in respect to that of the Bchl a in free LH2 has been observed. By using the relation of distance-dependent long-range electron transfer rate in protein, the distance between the donor B850 and the acceptor TiO2 nanoparticle has been estimated, which is about 0.6 nm. The proposed method of assembling proteins onto wide-gap semiconductor nanoparticle can be a promising way to determine the role of the protein playing in biological electron transfer processes.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000182280100018
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/80427]
专题	中国科学院化学研究所
通讯作者	Weng, YX
作者单位	1.Chinese Acad Sci, Inst Phys, Beijing 100080, Peoples R China 2.Kwansei Gakuin Univ, Fac Sci, Sanda, Hyogo 6691337, Japan 3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100010, Peoples R China
推荐引用方式 GB/T 7714	Weng, YX,Zhang, L,Yang, J,et al. Distance-dependent long-range electron transfer in protein: a case study of photosynthetic bacterial light-harvesting antenna complex LH2 assembled on TiO2 nanoparticle by femto-second time-resolved spectroscopy[J]. ACTA BOTANICA SINICA,2003,45(4):488-493.
APA	Weng, YX.,Zhang, L.,Yang, J.,Quan, DH.,Wang, L.,...&Zhang, BW.(2003).Distance-dependent long-range electron transfer in protein: a case study of photosynthetic bacterial light-harvesting antenna complex LH2 assembled on TiO2 nanoparticle by femto-second time-resolved spectroscopy.ACTA BOTANICA SINICA,45(4),488-493.
MLA	Weng, YX,et al."Distance-dependent long-range electron transfer in protein: a case study of photosynthetic bacterial light-harvesting antenna complex LH2 assembled on TiO2 nanoparticle by femto-second time-resolved spectroscopy".ACTA BOTANICA SINICA 45.4(2003):488-493.