Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin

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	Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin
	Wang Lin 1; Li Chun-Yan 1; He Pan 2; Fu Li 1; Zhou Yan-Min 1; Chen Xue-Si 2
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	2011-07-10
卷号	32 期号:7 页码:1622-1628
关键词	Chitosan Microsphere Poly(Lactide-co-glycolide) Nano-hydroxyapatite Adrenomedullin
ISSN号	0251-0790
英文摘要	By tripolyphosphate (TPP) as cross-linker, chitosan microspheres (CMs) loaded with adrenomedullin (ADM) were prepared by an emulsion-ionic cross-linking method. Then, porous poly(lactide-co-glycolide) (PLGA)/nano-hydroxyapatite (nHA)/CMs scaffold was developed by thermally induced phase separation. Scanning electron microscope was used to analyze the characterization of the microspheres and the scaffold and the drug release from them was tested in vitro. Also, the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. MG63 cell line and HUVEC cell line were cultured for testing the bioactivity of the scaffold loading ADM. After the cells adhered on the scaffold, the cell proliferation and differentiation were analyzed by methyl thiazolyl tetrazolium(MTT) assay, fluorescent staining and alkaline phosphatase(ALP) assay. The results show that the diameter of the microspheres is well-distributed and the PLGA/nHA/CMs scaffold shows a typical microstructure of polymeric foam. The pores of the scaffold ranged from 30 mu m to 220 mu m and they were interconnected. The hemolytic rate of the biomaterial was less than 5%, which was in line with national standard of medical materials. This study confirmed that PLGA/nHA/CMs/ADM and PLGA/nHA/CMs scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells and also could stimulate the differentiation of osteoblast-like cells.
语种	英语
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000293303800032
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/71913]
专题	中国科学院化学研究所
通讯作者	Zhou Yan-Min
作者单位	1.Jilin Univ, Stomatol Hosp, Implant Ctr, Changchun 130021, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Wang Lin,Li Chun-Yan,He Pan,et al. Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2011,32(7):1622-1628.
APA	Wang Lin,Li Chun-Yan,He Pan,Fu Li,Zhou Yan-Min,&Chen Xue-Si.(2011).Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,32(7),1622-1628.
MLA	Wang Lin,et al."Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 32.7(2011):1622-1628.