Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells

doi:10.1039/b904476k

CORC > 化学研究所 > 中国科学院化学研究所

	Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells
	Zhao, Zilong 1; Xu, Li 1; Shi, Xiaoli 1; Tan, Weihong 2; Fang, Xiaohong 1; Shangguan, Dihua 1
刊名	ANALYST
	2009
卷号	134 期号:9 页码:1808-1814
ISSN号	0003-2654
DOI	10.1039/b904476k
英文摘要	In this work, we have developed new aptamer probes for non-small cell lung cancer (NSCLC) by directing the aptamer selection process against the living cells of adenocarcinoma, the most common subtype of NSCLC. A panel of single-stranded DNA (ssDNA) aptamers were generated and evaluated for adenocarcinoma cell recognition. The aptamers bound to the adenocarcinoma cells with dissociation constants in the nanomolar range and the binding of the selected aptamers to the adenocarcinoma cells were significantly stronger than the other cancerous lung cells as well as other types of cancer cells. Moreover, the application of the aptamers to the clinical tissue section samples showed the differentiation of adenocarcinoma from normal lung tissue and other subtypes of lung cancer. The aptamers are expected to be new molecular probes for the investigation of the molecular bases of different NSCLC subtypes and their biological heterogeneity, which is valuable for advancing NSCLC diagnosis and treatment.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000269063100010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67827]
专题	中国科学院化学研究所
通讯作者	Fang, Xiaohong
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Florida, Dept Chem, Shands Canc Ctr, Gainesville, FL 32601 USA
推荐引用方式 GB/T 7714	Zhao, Zilong,Xu, Li,Shi, Xiaoli,et al. Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells[J]. ANALYST,2009,134(9):1808-1814.
APA	Zhao, Zilong,Xu, Li,Shi, Xiaoli,Tan, Weihong,Fang, Xiaohong,&Shangguan, Dihua.(2009).Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells.ANALYST,134(9),1808-1814.
MLA	Zhao, Zilong,et al."Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells".ANALYST 134.9(2009):1808-1814.