Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template

doi:10.1016/j.bios.2009.01.027

CORC > 化学研究所 > 中国科学院化学研究所

	Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template
	Hua, Zhendong; Zhou, Shuang; Zhao, Meiping
刊名	BIOSENSORS & BIOELECTRONICS
	2009-11-15
卷号	25 期号:3 页码:615-622
关键词	Surface Imprinting Hydrogel Bovine Serum Albumin Silica Microsphere
ISSN号	0956-5663
DOI	10.1016/j.bios.2009.01.027
英文摘要	Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat Of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K(a)) of 2.2 x 10(5) Lmol(-1) and a maximum binding capacity (Q(max)) of 27.3 mg g(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3 h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000271931900014
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66371]
专题	中国科学院化学研究所
通讯作者	Zhao, Meiping
作者单位	Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Hua, Zhendong,Zhou, Shuang,Zhao, Meiping. Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template[J]. BIOSENSORS & BIOELECTRONICS,2009,25(3):615-622.
APA	Hua, Zhendong,Zhou, Shuang,&Zhao, Meiping.(2009).Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template.BIOSENSORS & BIOELECTRONICS,25(3),615-622.
MLA	Hua, Zhendong,et al."Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template".BIOSENSORS & BIOELECTRONICS 25.3(2009):615-622.