The effect of Cu2+ on the interaction between an antitumor drug-mitoxantrone and human serum albumin

doi:10.1016/j.molstruc.2008.04.049

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	The effect of Cu2+ on the interaction between an antitumor drug-mitoxantrone and human serum albumin
	Tian, Ming-Yue 1,2; Zhang, Xiu-Feng 1; Xie, Ling 1; Xiang, Jun-Feng 1; Tang, Ya-Lin 1; Zhao, Chang-Qi 2
刊名	JOURNAL OF MOLECULAR STRUCTURE
	2008-12-15
卷号	892 期号:1-3 页码:20-24
关键词	Mitoxantrone Human Serum Albumin Cu2++++ Fluorescence Spectroscopy Circular Dichroism
ISSN号	0022-2860
DOI	10.1016/j.molstruc.2008.04.049
英文摘要	The studies on the interaction between HSA and drugs have been an interesting research field in life sciences, chemistry and clinical medicine. There are also many metal ions present in blood plasma, thus the research about the effect of metal ions on the interaction between drugs and plasma proteins is crucial. In this study, we have investigated the effect of a familiar metal ion-Cu2+ on the interaction between an antitumor drug-mitoxantrone (MTO) and human serum albumin (HSA) by using fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy and circular dichroism spectroscopy, for the first time. The results showed that the quenching efficiency of MTO to HSA is higher with Cu2+ than that without Cu2+. In the presence of Cu2+. the secondary structure of HSA was changed and the alpha-helix content was increased. The apparent association constant (K-A), the binding sites (n) and the spatial-distance (r) between MTO and HSA decreased. These results indicated that Cu2+ could affect the interaction between MTO and HSA by altering HSA molecular conformation. Further calculation indicated that the binding mode of Cu2+ in MTO-HSA system was likely to form Cu2+-HSA complex. (C) 2008 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000261711400004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/64893]
专题	中国科学院化学研究所
通讯作者	Tang, Ya-Lin
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Tian, Ming-Yue,Zhang, Xiu-Feng,Xie, Ling,et al. The effect of Cu2+ on the interaction between an antitumor drug-mitoxantrone and human serum albumin[J]. JOURNAL OF MOLECULAR STRUCTURE,2008,892(1-3):20-24.
APA	Tian, Ming-Yue,Zhang, Xiu-Feng,Xie, Ling,Xiang, Jun-Feng,Tang, Ya-Lin,&Zhao, Chang-Qi.(2008).The effect of Cu2+ on the interaction between an antitumor drug-mitoxantrone and human serum albumin.JOURNAL OF MOLECULAR STRUCTURE,892(1-3),20-24.
MLA	Tian, Ming-Yue,et al."The effect of Cu2+ on the interaction between an antitumor drug-mitoxantrone and human serum albumin".JOURNAL OF MOLECULAR STRUCTURE 892.1-3(2008):20-24.