Single molecule fluorescence imaging of thrombin aptamer folding

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	Single molecule fluorescence imaging of thrombin aptamer folding
	Ma Xin-Yong; Wang Jun; Chen Bo; Fang Xiao-Hong
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	2007-10-01
卷号	28 期号:10 页码:1852-1856
关键词	Single Molecule Fluorescence Electron Transfer G-quartet Thrombin Aptamer Folding
ISSN号	0251-0790
英文摘要	The folding of the thrombin DNA aptamer was studied with single molecule fluorescence imaging. The aptamer was singly labeled with TMR and immobilized on the glass surface. In the presence of K+, the folding of the aptamer resulted in the formation of G-quartets, thus brought TMR close to the guanosines. This led to the fluorescence decrease of TMR through the electron transfer between the dye and guanosines. From the fluorescence intensity of individual TMR, the distribution of aptamer molecules between the folded and random coil conformations was monitored at different concentrations of K+. The results show a new approach for the investigation of aptamer conformational change by electron transfer induced fluorescence quenching, which is complementary to the commonly used fluorescence resonance energy transfer in single molecule study.
语种	英语
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000250324400012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60409]
专题	中国科学院化学研究所
通讯作者	Fang Xiao-Hong
作者单位	Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Ma Xin-Yong,Wang Jun,Chen Bo,et al. Single molecule fluorescence imaging of thrombin aptamer folding[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(10):1852-1856.
APA	Ma Xin-Yong,Wang Jun,Chen Bo,&Fang Xiao-Hong.(2007).Single molecule fluorescence imaging of thrombin aptamer folding.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(10),1852-1856.
MLA	Ma Xin-Yong,et al."Single molecule fluorescence imaging of thrombin aptamer folding".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.10(2007):1852-1856.