Manufacturing and morphology structure of polylactide-type microtubules orientation-structured scaffolds

doi:10.1016/j.biomaterials.2006.05.028

CORC > 化学研究所 > 中国科学院化学研究所

	Manufacturing and morphology structure of polylactide-type microtubules orientation-structured scaffolds
	Yang, Fei ; Qu, Xue ; Cui, Wenjin ; Bei, Jianzhong ; Yu, Fangyuan ; Lu, Shibi ; Wang, Shenguo
刊名	BIOMATERIALS
	2006-10-01
卷号	27 期号:28 页码:4923-4933
关键词	Microtubules Orientation-structured Scaffold Thermal-induced Phase Separation Manufacturing Polylactide Plga Tissue Engineering
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2006.05.028
英文摘要	Tissue engineering using scaffold not only should have biodegradability and a certain 3D structure, but also its morphology structure should be mimetic to that of the repaired natural tissue. So to manufacture the scaffold with a biomimetic structure as the natural tissues is important. In this research, highly porous poly(L-lactic acid) (PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) scaffolds with microtubules orientation structure were designed and fabricated by using dioxane as solvent and an improved thermal-induced phase separation (TIPS) technique. All the factors which will affect solvent crystallization and microtubules orientation structure of the scaffold, such as the type of the solvent and polymer, concentration of the polymer solution, and temperature-gradient of the system have been studied carefully. So the porosity, diameter, tubular morphology and orientation of the microtubules could be controlled by adjusting the concentration of the polymer solution and temperature-gradient of the system. The scaffold with diameter of microtubules from 40 to 240 mu m and high porosity up to 96% could be obtained by adjusting temperature-gradient during the TIPS process. By increasing concentration of the polymer solution the regularity of the microtubular scaffold has been improved and the thickness of wall of the microtubules has been increased as well. In vitro cell culture results show that after the scaffolds have been improved by the ammonia plasma treatment and then collagen anchorage method, the human transparent cartilage cells H144, could be seeded deeply into the microtubules orientation-structured scaffolds and grew well there. (c) 2006 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000239617500011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60209]
专题	中国科学院化学研究所
通讯作者	Wang, Shenguo
作者单位	1.Chinese Acad Sci, Inst Chem, BNLMS State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China 2.Gen Hosp PLA, Inst Orthoped, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Yang, Fei,Qu, Xue,Cui, Wenjin,et al. Manufacturing and morphology structure of polylactide-type microtubules orientation-structured scaffolds[J]. BIOMATERIALS,2006,27(28):4923-4933.
APA	Yang, Fei.,Qu, Xue.,Cui, Wenjin.,Bei, Jianzhong.,Yu, Fangyuan.,...&Wang, Shenguo.(2006).Manufacturing and morphology structure of polylactide-type microtubules orientation-structured scaffolds.BIOMATERIALS,27(28),4923-4933.
MLA	Yang, Fei,et al."Manufacturing and morphology structure of polylactide-type microtubules orientation-structured scaffolds".BIOMATERIALS 27.28(2006):4923-4933.