Comparative investigation of structure characteristics of mixed beta-lactoglobulin and different chain-length phophatidylcholine monolayer at the air/water interface

doi:10.1016/j.colsurfa.2004.10.068

CORC > 化学研究所 > 中国科学院化学研究所

	Comparative investigation of structure characteristics of mixed beta-lactoglobulin and different chain-length phophatidylcholine monolayer at the air/water interface
	He, Q; Zhang, HJ; Tian, Y; Li, JB
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2005-05-05
卷号	257-58 页码:127-131
关键词	Complex Film Brewster Angle Microscope Lipid Protein Monolayer
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2004.10.068
英文摘要	The dynamic adsorption and penetration characteristics of beta-lactoglobulin into the monolayer of two different chain-length phosphatidyl-cholines (DSPC, DPPC) are systematically investigated using Brewster angle microscopy (BAM), fluorescence microscopy (FM) and film balance technique. It was found that all the two phosphatidylcholines can form stable complex films with the P-lactoglobulin. In the complex system of DPPC or DSPC/beta-lactoglobulin the lipid domains were observed again owing to the penetration of the protein. With the increase of surface pressure, the stripe and block domains appeared and gradually turned brighter. It has been considered that protein is squeezed out from the lipid monolayer and aggregated at interface. The critical surface pressure of the beta-lactoglobulin separated from DPPC or DSPC phase is increased when lipid has longer alkyl chain length. Therefore, the hydrophobic effect can be considered as a main factor to lead to the formation of a stabilized mixed lipid/protein film. (c) 2004 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000228321500025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/57273]
专题	中国科学院化学研究所
通讯作者	Li, JB
作者单位	Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci,Int Joint Lab, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	He, Q,Zhang, HJ,Tian, Y,et al. Comparative investigation of structure characteristics of mixed beta-lactoglobulin and different chain-length phophatidylcholine monolayer at the air/water interface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2005,257-58:127-131.
APA	He, Q,Zhang, HJ,Tian, Y,&Li, JB.(2005).Comparative investigation of structure characteristics of mixed beta-lactoglobulin and different chain-length phophatidylcholine monolayer at the air/water interface.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,257-58,127-131.
MLA	He, Q,et al."Comparative investigation of structure characteristics of mixed beta-lactoglobulin and different chain-length phophatidylcholine monolayer at the air/water interface".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 257-58(2005):127-131.