Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy

doi:10.1016/j.stam.2005.06.024

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	Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy
	Liu, YY ; Wang, PY ; Dou, SX ; Wang, WC ; Xie, P ; Yin, HW ; Lin, Y ; Jiang, YX ; Fang, XH ; Xi, XG
刊名	SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
	2005-10-01
卷号	6 期号:7 页码:842-847
关键词	Recq Helicase Dna Molecule Molecular Combing Fluorescence Microscopy Atomic Force Microscopy
ISSN号	1468-6996
DOI	10.1016/j.stam.2005.06.024
英文摘要	RecQ helicase-DNA interactions were directly visualized with fluorescence microscopy. DNA-RecQ complexes formed in binding and unwinding reaction were stretched onto the hydrophobic surface by molecular combing method. The complexes can be observed with fluorescence microscope because the DNA molecules were labeled with dye molecules of YOYO-1. The DNA binding and unwinding activity of RecQ helicase leads to reduced lengths of the observed DNA molecules. More direct observations with atomic force microscopy were also made. It was seen that RecQ is mainly monomeric both in solution and after binding to DNA. (c) 2005 Elsevier Ltd. All rights reserved.
语种	英语
出版者	NATL INST MATERIALS SCIENCE
WOS记录号	WOS:000234328200024
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/56701]
专题	中国科学院化学研究所
通讯作者	Wang, PY
作者单位	1.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 3.Ecole Normale Super, CNRS, UMR 8113, Lab Biotechnol & Pharmacol Genet Appl, F-94235 Cachan, France
推荐引用方式 GB/T 7714	Liu, YY,Wang, PY,Dou, SX,et al. Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy[J]. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,2005,6(7):842-847.
APA	Liu, YY.,Wang, PY.,Dou, SX.,Wang, WC.,Xie, P.,...&Xi, XG.(2005).Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy.SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,6(7),842-847.
MLA	Liu, YY,et al."Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy".SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 6.7(2005):842-847.