Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment

doi:10.1016/j.talanta.2014.01.009

CORC > 化学研究所 > 中国科学院化学研究所

	Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment
	Meng, Lingchen; Fang, Ziyuan; Lin, Jian; Li, Meixian; Zhu, Zhiwei
刊名	TALANTA
	2014-04-01
卷号	121 页码:205-209
关键词	Capillary Electrophoresis Copper Edta Hela Cells
ISSN号	0039-9140
DOI	10.1016/j.talanta.2014.01.009
英文摘要	A new separation system of capillary electrophoresis (CE1) for the highly sensitive determination of copper was established by using ethylenediaminetetraacetic acid (EDTA) as a complexing agent and employing cetyltrimethylammonium chloride (CTAC) as a capillary inner wall modifier. Benefitted from the combination of field-enhanced sample injection (FESI) method, a limit of detection (LOD) of 2.7 nM was obtained, which was much lower than that of the conventional methods. This made it possible to determine trace copper in HeLa cell only by a simple cell extraction (CE2) treatment. Two copper-extraction methods acid-hydrolysis and freeze-thaw were compared. Limited by the requirement of low ion strength in FESI, only the extract using freeze-thaw could be successfully applied in the determination. The effectiveness assessment of this CE2-FESI method was adopted by inductively coupled plasma-atomic emission spectrometry (ICP-AES) as a gold standard. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000333791500027
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52733]
专题	中国科学院化学研究所
通讯作者	Zhu, Zhiwei
作者单位	Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Meng, Lingchen,Fang, Ziyuan,Lin, Jian,et al. Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment[J]. TALANTA,2014,121:205-209.
APA	Meng, Lingchen,Fang, Ziyuan,Lin, Jian,Li, Meixian,&Zhu, Zhiwei.(2014).Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment.TALANTA,121,205-209.
MLA	Meng, Lingchen,et al."Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment".TALANTA 121(2014):205-209.