Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by gamma-irradiation

doi:10.1002/jbm.a.34938

CORC > 化学研究所 > 中国科学院化学研究所

	Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by gamma-irradiation
	Zhang, Xiangmei 1,2; Zhang, Yaqing 1,2; Chen, Wenqiang 2; Xu, Ling 1,2,3; Wei, Shicheng 2,4; Zheng, Yufeng 2; Zhai, Maolin 5
刊名	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
	2014-08-01
卷号	102 期号:8 页码:2669-2679
关键词	Collagen Hydrogel Radiation Crosslinking Contraction Fibroblasts
ISSN号	1549-3296
DOI	10.1002/jbm.a.34938
英文摘要	Collagen hydrogels exhibited a contractile trend in simulated body fluid. In this study, the internal pore architecture and mechanical properties of collagen hydrogel prepared by radiation crosslinking was evaluated during contraction, and the effect of contractile collagen hydrogels on the biological behavior of fibroblasts were investigated in vitro, such as viability, proliferation, morphology, apoptosis, cycle, and stress fiber. The results showed that accompany with contraction of collagen hydrogel, the pore diameter of the hydrogels decreased and compressive modulus increased. However, fibroblasts can grow on contractile collagen hydrogels. Indeed, collagen hydrogel contracted from circumference to the interior, which retard the spreading of fibroblasts on the dynamic substrate and interrupted the initial attachment of the cell. However, contraction of collagen hydrogel had not only significant influence on the L929 cell proliferation, but also accelerated the apoptosis. Cell cycle analysis showed that contractile collagen hydrogel may promote cell cycle from G(0)/G(1) phase to S phase, and DNA synthesis and cell proliferation were enhanced, but which may be different in contraction process. Therefore, as a scaffold for tissue engineering, the strategy for inhibition of the contraction of collagen hydrogel should be taken into account. (C) 2013 Wiley Periodicals, Inc.
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000339964300020
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/51357]
专题	中国科学院化学研究所
通讯作者	Xu, Ling
作者单位	1.Peking Univ, Beijing Key Lab Solid Waste Utilizat & Management, Coll Engn, Beijing 100871, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China 3.Peking Univ, ShenZhen Inst, Shenzhen Key Lab Polymer Sci, Shenzhen 518057, Peoples R China 4.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China 5.Peking Univ, Beijing Natl Lab Mol Sci, Dept Appl Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiangmei,Zhang, Yaqing,Chen, Wenqiang,et al. Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by gamma-irradiation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2014,102(8):2669-2679.
APA	Zhang, Xiangmei.,Zhang, Yaqing.,Chen, Wenqiang.,Xu, Ling.,Wei, Shicheng.,...&Zhai, Maolin.(2014).Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by gamma-irradiation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,102(8),2669-2679.
MLA	Zhang, Xiangmei,et al."Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by gamma-irradiation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 102.8(2014):2669-2679.