Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

doi:10.3866/PKU.WHXB201208161

CORC > 化学研究所 > 中国科学院化学研究所

	Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging
	Fu Xiao-Yi 1,2; Shao Guang-Sheng 1; Han Rong-Cheng 1; Ma Yan 1; Xue Fu-Min 1; Yang Fan 3; Fu Li-Min 3; Zhang Jian-Ping 3; Wang Yuan 1
刊名	ACTA PHYSICO-CHIMICA SINICA
	2012-10-01
卷号	28 期号:10 页码:2480-2486
关键词	Luminescence Europium Complex Two-photon Excitation Bio-nanoprobe Cell Imaging
ISSN号	1000-6818
DOI	10.3866/PKU.WHXB201208161
英文摘要	Luminescent nanospheres (EuPHS, d(av)=45 nm) containing 40% (w) of Eu(tta)(3)dpbt (tta=thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)(3)dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their good dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu3+ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu3+ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu3+ luminescence was 0.31 (lambda(ex)=415 nm, T=23 degrees C), and the two-photon excitation (TPE) action cross section was 5.0x10(5) GM (1 GM=10(-50) cm(4).s.photon(-1).particle(-1)) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.
语种	英语
出版者	PEKING UNIV PRESS
WOS记录号	WOS:000310181300030
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/48765]
专题	中国科学院化学研究所
通讯作者	Wang Yuan
作者单位	1.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.S China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510640, Peoples R China 3.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China
推荐引用方式 GB/T 7714	Fu Xiao-Yi,Shao Guang-Sheng,Han Rong-Cheng,et al. Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging[J]. ACTA PHYSICO-CHIMICA SINICA,2012,28(10):2480-2486.
APA	Fu Xiao-Yi.,Shao Guang-Sheng.,Han Rong-Cheng.,Ma Yan.,Xue Fu-Min.,...&Wang Yuan.(2012).Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging.ACTA PHYSICO-CHIMICA SINICA,28(10),2480-2486.
MLA	Fu Xiao-Yi,et al."Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging".ACTA PHYSICO-CHIMICA SINICA 28.10(2012):2480-2486.