Single-Molecule Fluorescence Imaging in Living Cells

doi:10.1146/annurev-physchem-040412-110127

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	Single-Molecule Fluorescence Imaging in Living Cells
	Xia, Tie; Li, Nan; Fang, Xiaohong; Johnson, MA; Martinez, TJ
刊名	ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64
	2013
卷号	64 页码:459-480
关键词	Fluorescence Microscopy Stoichiometry Single-biomolecule Kinetics Mobility Superresolution Imaging
ISSN号	0066-426X
DOI	10.1146/annurev-physchem-040412-110127
英文摘要	The transition of single-molecule fluorescence detection and imaging from in vitro to living cells has greatly enriched our knowledge on the behavior of single biomolecules in their native environments and their roles in cellular processes. Here we review recent advances of single-molecule biophysical approaches to live-cell studies based on fluorescence imaging. We start by discussing the practical considerations in designing single-molecule fluorescence imaging in cells, including the choice of fluorescent probes, labeling methods, instrumentation, and imaging techniques. We then describe representative examples in detail to illustrate the physicochemical parameters that can be obtained by imaging individually labeled biomolecules in cells and what can be learned from such characterizations.
语种	英语
出版者	ANNUAL REVIEWS
WOS记录号	WOS:000321771600021
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46683]
专题	中国科学院化学研究所
通讯作者	Xia, Tie
作者单位	Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xia, Tie,Li, Nan,Fang, Xiaohong,et al. Single-Molecule Fluorescence Imaging in Living Cells[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64,2013,64:459-480.
APA	Xia, Tie,Li, Nan,Fang, Xiaohong,Johnson, MA,&Martinez, TJ.(2013).Single-Molecule Fluorescence Imaging in Living Cells.ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64,64,459-480.
MLA	Xia, Tie,et al."Single-Molecule Fluorescence Imaging in Living Cells".ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64 64(2013):459-480.