ROS-Activated Ratiometric Fluorescent Polymeric Nanoparticles for Self-Reporting Drug Delivery

doi:10.1021/acsami.7b18438

CORC > 化学研究所 > 中国科学院化学研究所

	ROS-Activated Ratiometric Fluorescent Polymeric Nanoparticles for Self-Reporting Drug Delivery
	Zhang, Mei; Song, Cheng-Cheng; Su, Shan; Du, Fu-Sheng; Li, Zi-Chen
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-03-07
卷号	10 期号:9 页码:7798-7810
关键词	Oxidation-responsive Ratiometric Fluorescence Theranostic Polymer Self-reporting Drug Delivery
ISSN号	1944-8244
DOI	10.1021/acsami.7b18438
英文摘要	Reactive oxygen species (ROS)-responsive theranostic nanomedicines have attracted wide interest in recent years because ROS stress is implicated in some pathological disorders such as inflammatory diseases and cancers. In this article, we report a kind of innovative ROS-responsive theranostic polymeric nanoparticles that are able to load hydrophobic drugs and to fluorescently self-report the in vitro or intracellular drug release under ROS triggering. The fluorescent nanoparticles were formed by amphiphilic block copolymers consisting of a poly(ethylene glycol) (PEG) segment and an oxidation -responsive hydrophobic block. The copolymers with different hydrophobic block lengths were synthesized by the atom transfer radical polymerization of a phenylboronic ester-containing acrylic monomer with a small fraction of a ROS-activatable 1,8-naphthalimide-based fluorescent monomer, using PEG-Br as the macroinitiator. The copolymer nanoparticles were stable in neutral phosphate buffer but degraded upon H2O2 triggering, with the degradation rate depending on the hydrophobic block length and the concentration of H2O2. The degradation of nanoparticles was accompanied by a colorimetric change of the fluorophore from blue to green, which affords the nanoparticles the ability to detecting H2O2 by a ratiometric fluorescent approach. Moreover, the nanoparticles could encapsulate doxorubicin (DOX) and the H2O2-triggered DOX release was well associated with the change in ratiometric fluorescence. Confocal laser scanning microscope results reveal that the fluorescent nanoparticles were internalized into A549 cells through the endocytosis pathway. The ROS-stimulated degradation of the nanoparticles and intracellular DOX release and the fate of the degraded polymers could be monitored by ratiometric fluorescent imaging. Finally, the naked nanoparticles and the degradation products are cytocompatible, whereas the DOX-loaded ones exhibit concentration-dependent cytotoxicity. Of importance, the stimulation with exogenous H2O2 or lipopolysaccharide enhanced obviously the cell-killing capability of the DOX-loaded nanoparticles because of the ROS-enhanced intracellular DOX release.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000427204100030
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45841]
专题	中国科学院化学研究所
通讯作者	Du, Fu-Sheng; Li, Zi-Chen
作者单位	Peking Univ, Coll Chem & Mol Engn, Dept Polymer Sci & Engn, BNLMS,Key Lab Polymer Chem & Phys,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Mei,Song, Cheng-Cheng,Su, Shan,et al. ROS-Activated Ratiometric Fluorescent Polymeric Nanoparticles for Self-Reporting Drug Delivery[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(9):7798-7810.
APA	Zhang, Mei,Song, Cheng-Cheng,Su, Shan,Du, Fu-Sheng,&Li, Zi-Chen.(2018).ROS-Activated Ratiometric Fluorescent Polymeric Nanoparticles for Self-Reporting Drug Delivery.ACS APPLIED MATERIALS & INTERFACES,10(9),7798-7810.
MLA	Zhang, Mei,et al."ROS-Activated Ratiometric Fluorescent Polymeric Nanoparticles for Self-Reporting Drug Delivery".ACS APPLIED MATERIALS & INTERFACES 10.9(2018):7798-7810.