Silver nanoparticles as matrix for MALDI FTICR MS profiling and imaging of diverse lipids in brain

doi:10.1016/j.talanta.2017.11.067

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	Silver nanoparticles as matrix for MALDI FTICR MS profiling and imaging of diverse lipids in brain
	Guan, Ming 1,2; Zhang, Zhen 1,2; Li, Shilei 1,2; Liu, Jian'an 1; Liu, Lu 1,2; Yang, Hui 1,2; Zhang, Yangyang 1; Wang, Tie 1,2; Zhao, Zhenwen 1,2
刊名	TALANTA
	2018-03-01
卷号	179 页码:624-631
关键词	The Polyvinylpyrrolidone (Pvp) Capped Silver Nanoparticles (Agnps) Maldi Fticr Msi Lipids Metabolites Middle Cerebral Artery Occlusion (Mcao)
ISSN号	0039-9140
DOI	10.1016/j.talanta.2017.11.067
英文摘要	Owing to the diversity of lipids, profiling and imaging multiple classes of lipids in one analysis by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is a great challenge. In this work, polyvinylpyrrolidone (PVP) capped silver nanoparticles (AgNPs) was used as the matrix for MALDI MSI for the first time to simultaneously analyze 10 classes of lipids from the brain. This analysis included fatty acids and their derivatives, sterols, CPAs, LPA and PM, LPE and PEs, LPC and PCs, PS, Cers, SMs, and MAGs and DAGs, and other small metabolites. Owing to the abundant silver ions on the surface of PVP-capped AgNPs, compounds with poor ionization efficiency such as FM and sterols can be detected. The PVP-capped AgNPs based MALDI MSI analysis of mouse brain showed that lipid distributions in the substructures of the mouse brain can be connected with their biological functions. The brain lipids in rats with middle cerebral artery occlusion (MCAO) were also investigated. Most unsaturated FM, prostaglandins, CPAs, vitamin A, neuraminic acid, 5-OH-tryptophan and the K+ adducts of most phospholipids (PM, LPE, PEs, PCs, PS) and SMs were extremely down regulated in the ischemic region and saturated FA, Cers, hexanoylcarnitine, stearaldehyde, the Na+ adduct of phospholipids (LPA, PAs, LPE, PEs, LPC, PCs) and SMs were highly expressed in the damaged section. These novel findings could be very significant for elucidating the disease mechanism. MALDI MSI using PVP-capped AgNPs as a matrix can be a powerful tool in histopathology and pathology studies.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000424178000085
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45725]
专题	中国科学院化学研究所
通讯作者	Zhao, Zhenwen
作者单位	1.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing Natl Lab Mol Sci,Beijing Mass Spectrum Ct, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Guan, Ming,Zhang, Zhen,Li, Shilei,et al. Silver nanoparticles as matrix for MALDI FTICR MS profiling and imaging of diverse lipids in brain[J]. TALANTA,2018,179:624-631.
APA	Guan, Ming.,Zhang, Zhen.,Li, Shilei.,Liu, Jian'an.,Liu, Lu.,...&Zhao, Zhenwen.(2018).Silver nanoparticles as matrix for MALDI FTICR MS profiling and imaging of diverse lipids in brain.TALANTA,179,624-631.
MLA	Guan, Ming,et al."Silver nanoparticles as matrix for MALDI FTICR MS profiling and imaging of diverse lipids in brain".TALANTA 179(2018):624-631.