Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan

doi:10.1016/j.ijbiomac.2017.10.010

CORC > 化学研究所 > 中国科学院化学研究所

	Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan
	Li, Fangyuan 1; Zhou, Jiang 1; Xu, Ming 2; Yuan, Gu 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-02-01
卷号	107 页码:1474-1479
关键词	G-quadruplex C-myb Transcription Regulation
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2017.10.010
英文摘要	Our bioinformatics research shows that there are four G-rich sequences (S1-S4) in the upstream region of the transcription start site of c-Myb gene, and we have proved that these sequences have the ability to form G-quadruplex structures. This work mainly focuses on G-quadruplex function, recognition and transcription regulation in c-Myb gene, revealing a novel regulatory element in c-Myb proximal promoter region, and its transcription regulation by G-quadruplex binder. The research has identified that the enhancer effect in c-Myb transcription was primarily affected by the G-quadruplex formed by S1 sequence, and the up-regulation effect may due to the removal of repressive progress of MZF-1 by stabilizing G-quadruplex. Attentions were being paid to the development of G-quadruplex binders for selective recognition, and topotecan was found to have high binding affinity in vitro and could effectively affect the c-Myb transcription activities in cells. The regulation of G-quadruplex with binders in transcriptional, translational levels by Q-RT-PCR and western blot was in expectation of providing a strategy for gene expression modulation. In conclusion, our study revealed a G-quadruplex structure in c-Myb proximal promoter region, which was of great importance in the regulation of c-Myb function. (C) 2017 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000424062300012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45597]
专题	中国科学院化学研究所
通讯作者	Zhou, Jiang; Yuan, Gu
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Minist Educ,Dept Chem Biol, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China 2.Peking Univ, Dept Cardiol,Minist Hlth, Inst Vasc Med,Key Lab Cardiovasc Mol Biol & Regul, Hosp 3,Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Li, Fangyuan,Zhou, Jiang,Xu, Ming,et al. Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,107:1474-1479.
APA	Li, Fangyuan,Zhou, Jiang,Xu, Ming,&Yuan, Gu.(2018).Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,107,1474-1479.
MLA	Li, Fangyuan,et al."Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 107(2018):1474-1479.