Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells

doi:10.1039/c8sm01168k

CORC > 化学研究所 > 中国科学院化学研究所

	Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells
	Yin, Yudan 1; Chang, Haojing 1; Jing, Hairong 1; Zhang, Zexin 2; Yan, Dadong 3; Mann, Stephen 4; Liang, Dehai 1
刊名	SOFT MATTER
	2018-08-21
卷号	14 期号:31 页码:6514-6520
ISSN号	1744-683X
DOI	10.1039/c8sm01168k
英文摘要	Artificial protocells operating under non-equilibrium conditions offer a new approach to achieve dynamic features with life-like properties. Using coacervate micro-droplets comprising polylysine (PLL) and a short single-stranded oligonucleotide (ss-oligo) as a membrane-free protocell model, we demonstrate that circulation and vacuolization can occur simultaneously inside the droplet in the presence of an electric field. The circulation is driven by electrohydrodynamics and applies specifically to the major components of the protocell (PLL and ss-oligo). Significantly, under low electric fields (E = 10 V cm(-1)) the circulation regulates the movement of the vacuoles, while high levels of vacuolization produced at higher electric fields can deform or reshape the circulation. By taking advantage of the interplay between vacuolization and circulation, we achieve dynamic localization of an enzyme cascade reaction at specific droplet locations. In addition, the spatial distribution of the enzyme reaction is globalized throughout the droplet by tuning the coupling of the circulation and vacuolization processes. Overall, our work provides a new strategy to create non-equilibrium dynamic behaviors in molecularly crowded membrane-free synthetic protocells.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000442269000009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42489]
专题	中国科学院化学研究所
通讯作者	Zhang, Zexin; Liang, Dehai
作者单位	1.Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Polymer Chem & Phys, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Soochow Univ, Coll Chem Chem Engn & Mat Sci, State & Local Joint Engn Lab Novel Funct Polymer, Suzhou 215123, Peoples R China 3.Beijing Normal Univ, Dept Phys, Beijing 100875, Peoples R China 4.Univ Bristol, Sch Chem, Ctr Protolife Res, Bristol BS8 1TS, Avon, England
推荐引用方式 GB/T 7714	Yin, Yudan,Chang, Haojing,Jing, Hairong,et al. Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells[J]. SOFT MATTER,2018,14(31):6514-6520.
APA	Yin, Yudan.,Chang, Haojing.,Jing, Hairong.,Zhang, Zexin.,Yan, Dadong.,...&Liang, Dehai.(2018).Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells.SOFT MATTER,14(31),6514-6520.
MLA	Yin, Yudan,et al."Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells".SOFT MATTER 14.31(2018):6514-6520.