Prereduction-promoted enhanced growth of silver nanoparticles for ultrasensitive colorimetric detection of alkaline phosphatase and carbohydrate antigen 125

doi:10.1016/j.talanta.2018.06.064

CORC > 化学研究所 > 中国科学院化学研究所

	Prereduction-promoted enhanced growth of silver nanoparticles for ultrasensitive colorimetric detection of alkaline phosphatase and carbohydrate antigen 125
	Gao, Jing 1; Jia, Minna 1; Xu, Yingying 1; Zheng, Jimin 1; Shao, Na 1; Zhao, Meiping 2
刊名	TALANTA
	2018-11-01
卷号	189 页码:129-136
关键词	Silver Nanoparticles (Agnps) Colorimetric Method Ascorbic Acid (Aa) Prereduction Alkaline Phosphatase (Alp) Carbohydrate Antigen 125 (Ca125)
ISSN号	0039-9140
DOI	10.1016/j.talanta.2018.06.064
英文摘要	A highly sensitive colorimetric method for detection of alkaline phosphatase (ALP) and carbohydrate antigen (CA125) was developed, which was achieved by ascorbic acid (AA) - mediated enhanced growth of silver nanoparticles (AgNPs) promoted with NaBH4 as pre-reducing agent. With prereduction by NaBH4, the AA controlled growth of AgNPs was promoted and the localized surface plasmon resonance absorbance was much higher compared with that without prereduction. ALP could hydrolyze L-ascorbic acid 2-phosphate trisodium salt (AAP) to form AA, which could then reduce Ag+ to AgNPs resulting in the enhancement of localized surface plasmon resonance peak of AgNPs and accompanying the color change from colorless to bright yellow. Benefiting from the highly sensitive response of Ag+ to AA, the detection limit for ALP could be lowered to 0.003 U L-1, and the proposed plasmonic ELISA could achieve a low detection limit of 1.75 U mL(-1) for CA125. Moreover, this method was validated for the analysis of ALP and CA125 in human serum samples, giving results matched well with conventional method, demonstrating the potential application of the developed method in clinical diagnosis.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000442063900018
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42325]
专题	中国科学院化学研究所
通讯作者	Shao, Na; Zhao, Meiping
作者单位	1.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Gao, Jing,Jia, Minna,Xu, Yingying,et al. Prereduction-promoted enhanced growth of silver nanoparticles for ultrasensitive colorimetric detection of alkaline phosphatase and carbohydrate antigen 125[J]. TALANTA,2018,189:129-136.
APA	Gao, Jing,Jia, Minna,Xu, Yingying,Zheng, Jimin,Shao, Na,&Zhao, Meiping.(2018).Prereduction-promoted enhanced growth of silver nanoparticles for ultrasensitive colorimetric detection of alkaline phosphatase and carbohydrate antigen 125.TALANTA,189,129-136.
MLA	Gao, Jing,et al."Prereduction-promoted enhanced growth of silver nanoparticles for ultrasensitive colorimetric detection of alkaline phosphatase and carbohydrate antigen 125".TALANTA 189(2018):129-136.