Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA

doi:10.1021/acsami.8b07869

CORC > 化学研究所 > 中国科学院化学研究所

	Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA
	Jin, Ming 1; Liu, Xiangjun 2,3; Zhang, Xin 2,3; Wang, Linlin 2,3; Bing, Tao 2,3; Zhang, Nan 2; Zhang, Yun 1; Shangguan, Dihua 2,3
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-08-01
卷号	10 期号:30 页码:25166-25173
关键词	Carbon Dots Thiazole Orange Ratiometric Fluorescence Dna G-quadruplex
ISSN号	1944-8244
DOI	10.1021/acsami.8b07869
英文摘要	A new carbon dot (CD)-based nanoprobe for the ratiometric fluorescence detection of DNA was constructed in this work. Thiazole orange (TO), a specific organic small molecular probe toward DNA, is covalently linked to the surface of CDs, acting as the recognition element and the fluorescence response unit. In the absence of DNA, the nanoprobe only emitted the blue fluorescence of CDs, whereas TO was almost nonfluorescent. Upon addition of DNA, a turn-on emission at 530 nm appeared and gradually enhanced along with the increasing of the target DNA, whereas the fluorescence of CDs was unchanged, which realized the ratiometric detection of the target DNA. The CD-TO nanoprobe showed good selectivity to parallel G-quadruplex (G4) and double-stranded (ds) DNA over antiparallel G4 and single-stranded DNA. Moreover, the ratiometric fluorescence nanoprobe exhibited high sensitivity for ssab (a dsDNA) and c-myc (a parallel G4) with a low detection limit of 0.90 and 3.31 nM, respectively. Additionally, the G4/hemin peroxidase activity inhibition experiment demonstrated that CD-TO bound to the G4s through the end-stacking mode.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000440956000025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/41943]
专题	中国科学院化学研究所
通讯作者	Liu, Xiangjun; Zhang, Yun; Shangguan, Dihua
作者单位	1.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci,CAS Res,Inst Chem, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jin, Ming,Liu, Xiangjun,Zhang, Xin,et al. Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(30):25166-25173.
APA	Jin, Ming.,Liu, Xiangjun.,Zhang, Xin.,Wang, Linlin.,Bing, Tao.,...&Shangguan, Dihua.(2018).Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA.ACS APPLIED MATERIALS & INTERFACES,10(30),25166-25173.
MLA	Jin, Ming,et al."Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA".ACS APPLIED MATERIALS & INTERFACES 10.30(2018):25166-25173.