Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity

	Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity
	Chen, Chen ; Shao, Yun ; Tao, Yanduo ; Wen, Huaixiu
刊名	LWT-FOOD SCIENCE AND TECHNOLOGY
	2015-12-01
英文摘要	We used the Box Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X-1), microwave power (X-2) and water to raw material ratio (X-3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X-1 X-3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction, The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X-1 = 30.24 min, X-2 = 600.6 W and X-3 = 40 mL/g. The model was verified by modifying the optimal conditions (X-1 = 30 min, X-2 = 601 Wand X-3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 +/- 0.12% and 8.34 +/- 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI-H446 cells. (C) 2015 Elsevier Ltd. All rights reserved.; We used the Box Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X-1), microwave power (X-2) and water to raw material ratio (X-3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X-1 X-3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction, The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X-1 = 30.24 min, X-2 = 600.6 W and X-3 = 40 mL/g. The model was verified by modifying the optimal conditions (X-1 = 30 min, X-2 = 601 Wand X-3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 +/- 0.12% and 8.34 +/- 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI-H446 cells. (C) 2015 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/5489]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Chen,Shao, Yun,Tao, Yanduo,et al. Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2015.
APA	Chen, Chen,Shao, Yun,Tao, Yanduo,&Wen, Huaixiu.(2015).Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity.LWT-FOOD SCIENCE AND TECHNOLOGY.
MLA	Chen, Chen,et al."Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity".LWT-FOOD SCIENCE AND TECHNOLOGY (2015).