Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities

	Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
	Bi, Hongtao ; Gao, Tingting ; Liu, Dongbo ; Tai, Guihua ; Wei, Min ; Zhou, Yifa ; Wei, M (reprint author), NE Normal Univ, Sch Life Sci, Changchun 130022, Peoples R China.
刊名	CARBOHYDRATE POLYMERS ; Bi, HT; Gao, TT; Liu, DB; Tai, GH; Wei, M; Zhou, YF.Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities,CARBOHYDRATE POLYMERS,2013,93(2):547-552
	2013-04-02
英文摘要	In previous study, an unbranched (1 -> 6)-beta-D-glucan with Mw 2.6 kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1 -> 6)-beta-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5 kDa (BIK2), 288.9 kDa (BIK10) and 175.5 kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four beta-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1 -> 6)-beta-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1 -> 6)-beta-D-glucan, and the effect of (1 -> 6)-beta-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1 -> 6)-beta-D-glucan, and indicate (1 -> 6)-beta-D-glucans from B. inquinans (Fries) are potential immunostimulating agents. (c) 2012 Elsevier Ltd. All rights reserved.; In previous study, an unbranched (1 -> 6)-beta-D-glucan with Mw 2.6 kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1 -> 6)-beta-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5 kDa (BIK2), 288.9 kDa (BIK10) and 175.5 kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four beta-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1 -> 6)-beta-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1 -> 6)-beta-D-glucan, and the effect of (1 -> 6)-beta-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1 -> 6)-beta-D-glucan, and indicate (1 -> 6)-beta-D-glucans from B. inquinans (Fries) are potential immunostimulating agents. (c) 2012 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/3950]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Bi, Hongtao,Gao, Tingting,Liu, Dongbo,et al. Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities[J]. CARBOHYDRATE POLYMERS, Bi, HT; Gao, TT; Liu, DB; Tai, GH; Wei, M; Zhou, YF.Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities,CARBOHYDRATE POLYMERS,2013,93(2):547-552,2013.
APA	Bi, Hongtao.,Gao, Tingting.,Liu, Dongbo.,Tai, Guihua.,Wei, Min.,...&Wei, M .(2013).Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities.CARBOHYDRATE POLYMERS.
MLA	Bi, Hongtao,et al."Structures of (1 -> 6)-beta-D-glucans from Bulgaria inquinans (Fries) and their immunological activities".CARBOHYDRATE POLYMERS (2013).