QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY

	QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY
	Jing, Nianhua ; Li, Guoliang ; Sun, Zhiwei ; You, Jinmao
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES ; Shi, Y; Baumann, F; Ma, Y; Song, C; Kuhn, P; Scholten, T; He, JS.QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(13):1882-1894
	2012
英文摘要	Fatty acids (FA) are the basic and indispensable components in medicinal herbs. In the present work, a selective and sensitive method based on pre-column derivatization for the qualitative and quantitative analysis of FA from three snow lotus species (S. involucrata, S. laniceps, and S. medusa) was developed, and a comparative study based on the FA contents was carried out. FA were determined by HPLC with fluorescence detection, using 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent and were identified with post-column APCI-MS. FA could be easily and quickly tagged by BAETS at 95 degrees C in the presence of K2CO3 catalyst in N, N'-dimethylformamide (DMF). All validation procedures including linearity, limits of detection and quantification, precision, accuracy, ruggedness, and robustness were performed, and the results showed that the novel method exhibited excellent reproducibility and applicability. This proposed method was successfully applied to the analysis of total fatty acids (FA) and free fatty acids (FA) from snow lotus species. The content of FA in S. laniceps was significantly lower in comparison to the other two species that had similar FA content. Furthermore, this developed method exhibited a powerful potential for accurate detection of FA from other medicinal herbs or food stuff.; Fatty acids (FA) are the basic and indispensable components in medicinal herbs. In the present work, a selective and sensitive method based on pre-column derivatization for the qualitative and quantitative analysis of FA from three snow lotus species (S. involucrata, S. laniceps, and S. medusa) was developed, and a comparative study based on the FA contents was carried out. FA were determined by HPLC with fluorescence detection, using 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent and were identified with post-column APCI-MS. FA could be easily and quickly tagged by BAETS at 95 degrees C in the presence of K2CO3 catalyst in N, N'-dimethylformamide (DMF). All validation procedures including linearity, limits of detection and quantification, precision, accuracy, ruggedness, and robustness were performed, and the results showed that the novel method exhibited excellent reproducibility and applicability. This proposed method was successfully applied to the analysis of total fatty acids (FA) and free fatty acids (FA) from snow lotus species. The content of FA in S. laniceps was significantly lower in comparison to the other two species that had similar FA content. Furthermore, this developed method exhibited a powerful potential for accurate detection of FA from other medicinal herbs or food stuff.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/3746]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jing, Nianhua,Li, Guoliang,Sun, Zhiwei,et al. QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Shi, Y; Baumann, F; Ma, Y; Song, C; Kuhn, P; Scholten, T; He, JS.QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(13):1882-1894,2012.
APA	Jing, Nianhua,Li, Guoliang,Sun, Zhiwei,&You, Jinmao.(2012).QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA	Jing, Nianhua,et al."QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOW LOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2012).