Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2

doi:10.1109/ICSENS.2018.8589938

	Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2
	Matassan, Nur Diyanah 1; Rizwan, Mohammad 1; Mohd-Naim, Noor Faizah 1,2; Tlili, Chaker2 ; Ahmed, Minhaz Uddin 1
	2018
会议日期	October 28, 2018 - October 31, 2018
会议地点	New Delhi, India
DOI	10.1109/ICSENS.2018.8589938
英文摘要	An electrochemical aptasensor platform was developed by using amine-functionalized aptamer that was incorporated onto carbon-printed electrode and modified with graphene nanoplatelets for the detection of lipocalin-2 (LCN-2) protein. The aptamers were covalently bound to the graphene nanoplatelets surface via 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry and were used as the molecular recognition element for the protein target. Successful interaction between aptamer and LCN-2 protein was ascertained via electrochemical probe, ferro- and ferri-cyanide, Fe(CN)63-/4-, and monitored by means of voltammetric analyses. The developed LCN-2 aptasensor achieved sub-picomolar detection of protein, with the detection limit determined as 0.07 pg ml-1, and a dynamic linear range of 0.1 to 1000 pg ml-1. © 2018 IEEE.
会议录	17th IEEE SENSORS Conference, SENSORS 2018
语种	英语
电子版国际标准刊号	21689229
ISSN号	19300395
内容类型	会议论文
源URL	[http://119.78.100.138/handle/2HOD01W0/7970]
专题	中国科学院重庆绿色智能技术研究院
作者单位	1.Faculty of Science, IBiosensors and Biotechnology Laboratory, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong; BE1410, Brunei; 2.Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Chongqing, China
推荐引用方式 GB/T 7714	Matassan, Nur Diyanah,Rizwan, Mohammad,Mohd-Naim, Noor Faizah,et al. Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2[C]. 见:. New Delhi, India. October 28, 2018 - October 31, 2018.