Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha | |
Zhao, Mei-Li; Ni, Jun; Chen, Mao-Sheng; Xu, Zeng-Fu | |
刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
2019 | |
卷号 | 20期号:x页码:- |
关键词 | TREHALOSE 6-PHOSPHATE GENE-EXPRESSION DIFFERENT-FORMS METABOLISM BIOSYNTHESIS GROWTH TOLERANCE EVOLUTION STRESS SUGAR |
ISSN号 | 1422-0067 |
DOI | 10.3390/ijms20092165 |
英文摘要 | Trehalose-6-phosphate (T6P) phosphatase (TPP), a dephosphorylating enzyme, catalyzes the dephosphorylation of T6P, generating trehalose. In Jatropha, we found six members of the TPP family. Five of them JcTPPA, JcTPPC, JcTPPD, JcTPPG, and JcTPPJ are highly expressed in female flowers or male flowers, or both, suggesting that members of the JcTPP family may participate in flower development in Jatropha. The wide expression of JcTPPJ gene in various organs implied its versatile roles and thus was chosen for unraveling its biological functions during developmental process. We constructed an overexpression vector of JcTPPJ cDNA driven by the cauliflower mosaic virus (CaMV) 35S promoter for genetic transformation. Compared with control Arabidopsis plants, 35S:JcTPPJ transgenic Arabidopsis plants presented greater sucrose contents in their inflorescences and displayed late-flowering and heterostylous phenotypes. Exogenous application of sucrose to the inflorescence buds of wild-type Arabidopsis repressed the development of the perianth and filaments, with a phenocopy of the 35S:JcTPPJ transgenic Arabidopsis. These results suggested that the significantly increased sucrose level in the inflorescence caused (or induced) by JcTTPJ overexpression, was responsible for the formation of heterostylous flower phenotype. However, 35S:JcTPPJ transgenic Jatropha displayed no obvious phenotypic changes, implying that JcTPPJ alone may not be sufficient for regulating flower development in Jatropha. Our results are helpful for understanding the function of TPPs, which may regulate flower organ development by manipulating the sucrose status in plants. |
学科主题 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000469753500112 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/11321] |
专题 | 西双版纳热带植物园_能源植物分子育种研究组 |
作者单位 | 1.[Zhao, Mei-Li 2.Ni, Jun 3.Chen, Mao-Sheng 4.Chinese Acad Sci, Innovat Acad Seed Design, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Plant Resources & Sustainable Us, Mengla 666303, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Mei-Li,Ni, Jun,Chen, Mao-Sheng,et al. Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(x):-. |
APA | Zhao, Mei-Li,Ni, Jun,Chen, Mao-Sheng,&Xu, Zeng-Fu.(2019).Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(x),-. |
MLA | Zhao, Mei-Li,et al."Ectopic Expression of Jatropha curcas TREHALOSE-6-PHOSPHATE PHOSPHATASE J Causes Late-Flowering and Heterostylous Phenotypes in Arabidopsis but not in Jatropha".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.x(2019):-. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论