The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink

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	The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink
	Zhuang, Guoqiang; Zhang, Yanfen; Ma, Anzhou; Liu, Wenzong; Bai, Zhihui; Zhuang, Xuliang
刊名	ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL
	2018
页码	-
ISSN号	1472-3646
文献子类	Article
英文摘要	Recently, a new oxygenic pathway has been proposed based on the disproportionation of NO with putative NO dismutase (Nod). In addition to a new process in nitrogen cycling, this process provides ecological advantages for the degradation of substrates in anaerobic conditions, which is of great significance for wastewater treatment. However, the Nod distribution in aquatic environments is rarely investigated. In this study, we obtained the nod genes with an abundance of 2.38 +/- 0.96 x 10(5) copies per gram of dry soil from the Zoige wetland and aligned the molecular characteristics in the corresponding Nod sequences. These Nod sequences were not only found existing in NC10 bacteria, but were also found forming some other clusters with Nod sequences from a WWTP reactor or contaminated aquifers. Moreover, a new subcluster in the aquifer-similar cluster was even dominant in the Zoige wetland and was named the Z-aquifer subcluster. Additionally, soils from the Zoige wetland showed a high potential rate (10.97 +/- 1.42 nmol of CO2 per gram of dry soil per day) for nitrite-dependent anaerobic methane oxidation (N-DAMO) with low abundance of NC10 bacteria, which may suggest a potential activity of Nod in other clusters when considering the dominance of the Z-aquifer subcluster Nod. In conclusion, we verified the occurrence of Nod in an alpine wetland for the first time and found a new subcluster to be dominant in the Zoige wetland. Moreover, this new subcluster of Nod may even be active in the N-DAMO process in this alpine wetland, which needs further study to confirm.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41303]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Zhuang, Guoqiang,Zhang, Yanfen,Ma, Anzhou,et al. The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink[J]. ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL,2018:-.
APA	Zhuang, Guoqiang,Zhang, Yanfen,Ma, Anzhou,Liu, Wenzong,Bai, Zhihui,&Zhuang, Xuliang.(2018).The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink.ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL,-.
MLA	Zhuang, Guoqiang,et al."The Occurrence of Putative Nitric Oxide Dismutase (Nod) in an Alpine Wetland with a New Dominant Subcluster and the Potential Ability for a Methane Sink".ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL (2018):-.