Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional
	Qu, Guangbo; Li, Yiling; He, Bin; Gao, Jiejun; Liu, Qian S.; Liu, Runzeng; Shi, Jianbo; Hu, Ligang; Jiang, Guibin
刊名	ENVIRONMENTAL POLLUTION
	2018-07-01
卷号	238 页码:213-221
关键词	Methylmercury Mercury chloride Differentially expressed proteins Alternative splicing SK-N-SH cells
ISSN号	0269-7491
文献子类	Article
英文摘要	The neurotoxic effects of methylmercury (MeHg) have been intensively studied. However, the molecular mechanisms responsible for the neurotoxicity of MeHg are not fully understood. To decipher these mechanisms, proteomic and high-throughput mRNA sequencing (RNA-seq) technique were utilized, comprehensively evaluating the cellular responses of human neuroblastoma SK-N-SH cells to MeHg exposure. Proteomic results revealed that MeHg exposure interfered with RNA splicing via splicesome, along with the known molecular mechanisms of mercury-related neurotoxicity (e.g. oxidative stress, protein folding, immune system processes, and cytoskeletal organization). The effects of MeHg on RNA splicing were further verified using RNA-seq. Compared to control, a total of 658 aberrant RNA alternative splicing (AS) events were observed after MeHg exposure. Proteomics and RNA-seq results also demonstrated that mercury chloride (HgCl2) influenced the expression levels of several RNA splicing related proteins and 676 AS events compared to control. These results suggested that RNA splicing could be a new molecular mechanism involved in MeHg and HgCl2 neurotoxicity. (C) 2018 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40982]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Qu, Guangbo,Li, Yiling,He, Bin,et al. Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional[J]. ENVIRONMENTAL POLLUTION,2018,238:213-221.
APA	Qu, Guangbo.,Li, Yiling.,He, Bin.,Gao, Jiejun.,Liu, Qian S..,...&Jiang, Guibin.(2018).Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional.ENVIRONMENTAL POLLUTION,238,213-221.
MLA	Qu, Guangbo,et al."Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional".ENVIRONMENTAL POLLUTION 238(2018):213-221.