Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells
	Sha, Rui; Zhao, Bin; Xu, Li; Chen, Yangsheng; Wang, Lingyun; Xia, Yingjie; Li, Yunping; Xie, Heidi Q.; Xu, Tuan
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2018
卷号	63 页码:260-267
关键词	2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) MicroRNA Acetylcholinesterase (AChE) Neuron Transcription regulation Post-transcriptional regulation
ISSN号	1001-0742
文献子类	Article
英文摘要	Dioxin can cause a series of neural toxicological effects. MicroRNAs (miRs) play important roles in regulating nervous systemfunction andmediating cellular responses to environmental pollutants, such as dioxin. Hsa-miR-146b-5p appears to be involved in neurodegenerative diseases and brain tumors. However, little is known about effects of dioxin on the expression of hsa-miR-146b-5p. We found that the hsa-miR-146b-5p expression and its promoter activity were significantly increased in dioxin treated SK-N-SH cells, a human-derived neuroblastoma cell line. Potential roles of hsa-miR-146b-5p in mediating neural toxicological effects of dioxin may be due to the regulation of certain target genes. We further confirmed that hsa-miR-146b-5p significantly suppressed acetylcholinesterase (AChE) activity and targeted the 3'-untranslated region of the AChE T subunit, which has been down-regulated in dioxin treated SK-N-SH cells. Functional bioinformatic analysis showed that the known and predicted target genes of hsa-miR-146b-5p were involved in some brain functions or cyto-toxicities related to known dioxin effects, including synapse transmission, in which AChE may serve as a responsive gene for mediating the effect. (C) 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40921]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Sha, Rui,Zhao, Bin,Xu, Li,et al. Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2018,63:260-267.
APA	Sha, Rui.,Zhao, Bin.,Xu, Li.,Chen, Yangsheng.,Wang, Lingyun.,...&Xu, Tuan.(2018).Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells.JOURNAL OF ENVIRONMENTAL SCIENCES,63,260-267.
MLA	Sha, Rui,et al."Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells".JOURNAL OF ENVIRONMENTAL SCIENCES 63(2018):260-267.