Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe
	Wang, Yue; Gao, Min; Chen, Qingguo; Yu, Fabiao; Jiang, Guibin; Chen, Lingxin
刊名	ANALYTICAL CHEMISTRY
	2018-08-21
卷号	90 期号:16 页码:9769-9778
ISSN号	0003-2700
文献子类	Article
英文摘要	As a cytotoxic heavy metal ion, mercury(II) ion (Hg2+) induces severe oxidative stress and further results in physiological dysfunction. Although mercury poisoning can be treated with many drugs, such as sodium selenite, the therapeutic effect is relatively poor, and it seems that the damage to human health continues. However, the interpretation for the pathogenesis has not been clarified yet. We supposed that the reason is attributed to Hg2+-caused intracellular oxidative stress. To confirm our hypothesis, we strived to design a three-channel ratio fluorescent probe, HCy-SeH, for superoxide anion (O-2(center dot-)) and Hg2+ combined detection. O-2(center dot-) is a vital precursor for other reactive oxygen species (ROS), which is involved in many physiological and pathological processes. However, until now there is no efficient chemical tool for O-2(center dot-) and Hg2+ combined detection in cells and in vivo. The fluorescence response of our probe is initiated by a hydrogen abstraction reaction from the hydrocyanine fluorophore moiety. Once oxidized by O-2(center dot-), HCy-SeH recovers its pi-conjugated system back to a heptamethine cyanine derivative, Cy-SeH. Cy-SeH coexists with its conjugate base,Cy=Se. One emits red fluorescence, and the other one emits green fluorescence. The response unit, -SeH, can trap Hg2+ via a Se-Hg antagonism reaction to afford an orange-emitting final product, Keto-Cy. The probe offers high selectivity and sensitivity toward O-2(center dot-). and Hg2+. When applied for O-2(center dot-) and Hg2+ detection in HEK 293 cells, the imaging results indicate that our probe can provide a combined response for O-2(center dot-). and Hg2+ in real time and in situ. Flow cytometry analysis is well-consistent with the results from fluorescence imaging. When applied to image O-2(center dot-) and Hg2+ in mice models, we find that Hg2+ dominantly accumulates in the kidney and induces a burst of O-2(center dot-). We confirm that chronic mercurialism can cause severe oxidative damage and renal fibrosis. HCy-SeH further provides a new information that, even when intracellular Hg2+ has been antagonized, the outbreak of O-2(center dot-). caused by mercury poisoning still lasts.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40883]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Yue,Gao, Min,Chen, Qingguo,et al. Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2018,90(16):9769-9778.
APA	Wang, Yue,Gao, Min,Chen, Qingguo,Yu, Fabiao,Jiang, Guibin,&Chen, Lingxin.(2018).Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe.ANALYTICAL CHEMISTRY,90(16),9769-9778.
MLA	Wang, Yue,et al."Associated Detection of Superoxide Anion and Mercury(II) under Chronic Mercury Exposure in Cells and Mice Models via a Three-Channel Fluorescent Probe".ANALYTICAL CHEMISTRY 90.16(2018):9769-9778.