2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells
	Li, Yunping; Fu, Hualing; Xie, Heidi Q.; Chen, Yangsheng; Xu, Tuan; Xia, Yingjie; Luo, Yali; Xu, Li; Tsim, Karl W. K.; Zhao, Bin
刊名	ENVIRONMENTAL POLLUTION
	2018-04-01
卷号	235 页码:965-973
关键词	2,3,7,8-tetrachlorodibenzo-p-dioxin Acetylcholinesterase C2C12 cells Myogenic differentiation Aryl hydrocarbon receptor (AhR)
ISSN号	0269-7491
文献子类	Article
英文摘要	Dioxin-induced toxicities that affect the development of the motor system have been proposed since many years. However, cellular evidence and the molecular basis for the effects are limited. In this study, a cultured mouse myoblast cell line, C2C12, was utilized to examine the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on myogenic differentiation and expression of acetylcholinesterase (AChE), a neuromuscular transmission-related gene. The results showed that TCDD exposure at 10(-10) M repressed the myotube formation of C2C12 cells by disturbing the fusion process and suppressing the expression of myosin heavy chain, a myobute structural protein, and not by induction of cytotoxicity. Furthermore, TCDD dose dependently suppressed the transcriptional expression and enzymatic activity of AChE during the myogenic differentiation, particularly in the middle stage. However, the administration of aryl hydrocarbon receptor antagonists, CH223191 and alpha-naphthoflavone, did not completely reverse the TCDD-induced downregulation of muscular AChE during myogenic differentiation. These findings suggest that low dose exposure to dioxin may result in disturbances of muscle differentiation and neuromuscular transmission. (C) 2018 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40860]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Li, Yunping,Fu, Hualing,Xie, Heidi Q.,et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells[J]. ENVIRONMENTAL POLLUTION,2018,235:965-973.
APA	Li, Yunping.,Fu, Hualing.,Xie, Heidi Q..,Chen, Yangsheng.,Xu, Tuan.,...&Zhao, Bin.(2018).2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells.ENVIRONMENTAL POLLUTION,235,965-973.
MLA	Li, Yunping,et al."2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells".ENVIRONMENTAL POLLUTION 235(2018):965-973.