Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage

doi:10.3389/fpls.2018.00221

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage
	Gu, Ruiting 1,2,3; Zhou, Yi1,2 ; Song, Xiaoyue 1,2,3; Xu, Shaochun1,2,3 ; Zhang, Xiaomei1,2 ; Lin, Haiying 4; Xu, Shuai 1,2,3; Yue, Shidong 1,2,3; Zhu, Shuyu 5
刊名	FRONTIERS IN PLANT SCIENCE
	2018-03-23
卷号	9 页码:13
关键词	desiccation morphology seagrass Ruppia sinensis seed storage salinity temperature
ISSN号	1664-462X
DOI	10.3389/fpls.2018.00221
通讯作者	Zhou, Yi(yizhou@qdio.ac.cn)
英文摘要	Seeds are important materials for the restoration of globally-threatened marine angiosperm (seagrass) populations. In this study, we investigated the differences between different Ruppia sinensis seed types and developed two feasible long-term R. sinensis seed storage methods. The ability of R. sinensis seeds to tolerate the short-term desiccation and extreme cold had been investigated. The tolerance of R. sinensis seeds to long-term exposure of high salinity, cold temperature, and desiccation had been considered as potential methods for long-term seed storage. Also, three morphological and nine physiological indices were measured and compared between two types of seeds: Shape L and Shape S. We found that: (1) wet storage at a salinity of 30-40 psu and 0 degrees C were the optimal long-term storage conditions, and the proportion of viable seeds reached over 90% after a storage period of 11 months since the seeds were collected from the reproductive shoots; (2) dry condition was not the optimal choice for long-term storage of R. sinensis seeds; however, storing seeds in a dry condition at 5 degrees C and 33 +/- 10% relative humidity for 9 months had a relatively high percentage (74.44 +/- 2.22%) of viable seeds, consequently desiccation exposure could also be an acceptable seed storage method; (3) R. sinensis seeds would lose vigor in the interaction of extreme cold (-27 degrees C) and desiccation; (4) there were significant differences in seed weight, seed curvature, and endocarp thickness between the two types of seeds. These findings provided fundamental physiological information for R. sinensis seeds and supported the long-term storage of its seeds. Our results may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.
资助项目	National Science and Technology Basic Work Program[2015FY110600] ; National Marine Public Welfare Research Project[201305043] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; National Natural Science Foundation of China[41176140/30972268] ; Key Research and Development Project of Shandong Province[2017GHY15111] ; Key Research Project of Frontier Sciences of CAS[QYZDB-SSW-DQC041-1] ; CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows[2016LH0032] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Taishan Scholars Program
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000428149000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/157587]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhou, Yi
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 4.Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing, Peoples R China 5.Yellow River Delta Natl Nat Reserve Management Bu, Dongying, Peoples R China
推荐引用方式 GB/T 7714	Gu, Ruiting,Zhou, Yi,Song, Xiaoyue,et al. Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage[J]. FRONTIERS IN PLANT SCIENCE,2018,9:13.
APA	Gu, Ruiting.,Zhou, Yi.,Song, Xiaoyue.,Xu, Shaochun.,Zhang, Xiaomei.,...&Zhu, Shuyu.(2018).Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage.FRONTIERS IN PLANT SCIENCE,9,13.
MLA	Gu, Ruiting,et al."Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage".FRONTIERS IN PLANT SCIENCE 9(2018):13.