A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas
Liu, Rui1; Cheng, Qi1,4; Song, Xiaorui2,3,5; Wang, Hao1; Wang, Xiudan1; Wang, Lingling3,5; Zhu, Beiwei4; Song, Linsheng2,3,5
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
2019-02-01
卷号91页码:132-142
关键词Ub-conjugating enzyme E2 Inflammatory response Cellular immunity Crassostrea gigas NF-kappa B pathway Ubiquitination
ISSN号0145-305X
DOI10.1016/j.dci.2018.10.014
通讯作者Wang, Hao() ; Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要As an important post-translational protein modification, ubiquitination has been demonstrated to play vitalrole in immune response of vertebrates. Ubiquitin (Ub)-conjugating enzyme E2 is the "heart" of ubiquitination, which is responsible for Ub cellular signaling and substrate modification. In the present study, an Ub-conjugating enzyme E2 (designed as CgUbe2g1) was identified from oyster Crassostrea gigas, and its regulation in the immune response against lipopolysaccharide (LPS) stimulation was investigated. CgUbe2g1 encoded a polypeptide of 168 amino acids with the predicted molecular mass of 19.20 kDa and contained conserved catalytic 'Ube domains. It shared a higher similarity with the known UBC2G1 type E2s and was closely clustered with the type E2s identified from invertebrates in the phylogenetic assay. The mRNA transcripts of CgUbe2g1 were mainly distributed in hemocyte, mantle, hepatopancreas and male gonad of C. gigas. CgUbe2g1 protein was found to be colocalized with Ub around the nucleus of oyster hemocyte. The recombinant CgUbe2g1 protein (rCgUbe2g1) could activate the ubiquitination in vitro by binding both activated and un-activated Ub. The expressions of inflammation-related factors TNF-alpha and NF-kappa B in CgUbe2g1 transfected cells were both significantly up-regulated after LPS stimulation, which were 12.9-fold at 3h (p < 0.01) and 2.3-fold at 6h (p < 0.01) of that in negative control group, respectively. The phagocytic rate of hemocyte and the ROS level in hemocyte were both significantly decreased (p < 0.01), while the apoptosis rate was significantly increased (p < 0.01) after CgUbe2g1 mRNA was interfered. These results demonstrated that Ub-conjugating enzyme CgUbe2g1 was involved in the innate immune response of oyster against invading pathogen, which might play important roles in the activation of inflammatory response and regulation of cellular immune response.
资助项目National Science Foundation of China[U1706204] ; Aoshan Talent Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Modern Agro-industry Technology Research System, Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Dalian High Level Talent Innovation Support Program[2015R020]
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000451492900015
内容类型期刊论文
源URL[http://ir.qdio.ac.cn/handle/337002/156148]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Hao; Song, Linsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
4.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
5.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
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Liu, Rui,Cheng, Qi,Song, Xiaorui,et al. A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,91:132-142.
APA Liu, Rui.,Cheng, Qi.,Song, Xiaorui.,Wang, Hao.,Wang, Xiudan.,...&Song, Linsheng.(2019).A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,91,132-142.
MLA Liu, Rui,et al."A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 91(2019):132-142.
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