Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)

doi:10.1007/s13131-019-1351-4

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)
	Wang, Yu 1,3,6; Zhang, Shoudu 4; Zhang, Guofan 1,2,5; Cong, Rihao 1,2,5; Li, Li 1,5
刊名	ACTA OCEANOLOGICA SINICA
	2019-02-01
卷号	38 期号:2 页码:44-51
关键词	Argopecten irradians inbreeding depression differentially expressed genes transcriptome
ISSN号	0253-505X
DOI	10.1007/s13131-019-1351-4
通讯作者	Cong, Rihao(rhcong@qdio.ac.cn) ; Li, Li(lili@qdio.ac.cn)
英文摘要	Understanding the effects of inbreeding is critical for the long-term viability of shellfish breeding and for understanding inbreeding depression (ID) of the offspring of selfed individuals and full-sib crosses. The bay scallop (Argopecten irradians) is a simultaneous hermaphroditic marine bivalve with distinct male and female gonads and is a species with external fertilization. It is a canonical model invertebrate for analysis of the genetics of ID and evolution of outcrossing mating system. ID in bivalves is well documented with regard to the phenotype and molecular markers aspects, but little is known on how inbreeding affects genome-wide gene expression. Here, we compare the gene expression profiles of adductor muscle of A. irradians lines inbred to F=0.5 with those of its counterpart outbred lines which are originated from the same paternal individual. Inbred and outbred individuals have some differences in growth traits. The shell length and the total weight of inbred scallops are lower than the counterpart of outbred lines. We found that 1 175 genes were up-regulated while 1 390 were downregulated in inbred lines. Regarding components, differentially expressed genes (DEGs) were concentrated on sarcomeres, contractile filaments, and cell membranes. Among others, whereas regarding their function, the Gene Ontology (GO) was concentrated on transferase, phosphotransferase, peptidase, and others. The DEGs-enriched metabolic pathways mainly belonged to the oxidative phosphorylation pathway and apoptosis pathway. We used another two families to verify our bioinformatic results. The selfed and the outbred family originated from different ancestors, but the selfed family showed obvious ID. By using real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), we verified that DEGs with inbreeding are involved in the oxidative phosphorylation and cell apoptosis pathways, suggesting that inbreeding may affect energy metabolism and homeostasis.
资助项目	National Natural Science Foundation of China[30800842] ; National Natural Science Foundation of China[31502164] ; Program of the Chinese Academy of Science[ZKCX2-211] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000460726200005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155256]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Cong, Rihao; Li, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Tianjin Bohai Sea Fisheries Res Inst, Tianjin 300457, Peoples R China 4.Natl Oceanog Ctr, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 6.Tianjin Ocean Ranch Tech Engn Ctr, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yu,Zhang, Shoudu,Zhang, Guofan,et al. Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)[J]. ACTA OCEANOLOGICA SINICA,2019,38(2):44-51.
APA	Wang, Yu,Zhang, Shoudu,Zhang, Guofan,Cong, Rihao,&Li, Li.(2019).Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians).ACTA OCEANOLOGICA SINICA,38(2),44-51.
MLA	Wang, Yu,et al."Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)".ACTA OCEANOLOGICA SINICA 38.2(2019):44-51.