Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection

doi:10.1186/s12864-019-5614-4

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection
	Wang, Fuxuan 1,2,3; Li, Shihao 1,2,4; Xiang, Jianhai1,2 ; Li, Fuhua 1,2,4
刊名	BMC GENOMICS
	2019-03-28
卷号	20 页码:14
关键词	Litopenaeus vannamei Hemocytes WSSV Neuroendocrine-immune system
ISSN号	1471-2164
DOI	10.1186/s12864-019-5614-4
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	Background: Functional communications between nervous, endocrine and immune systems are well established in both vertebrates and invertebrates. Circulating hemocytes act as fundamental players in this crosstalk, whose functions are conserved during the evolution of the main groups of metazoans. However, the roles of the neuroendocrineimmune (NEI) system in shrimp hemocytes during pathogen infection remain largely unknown. Results: In this study, we sequenced six cDNA libraries prepared with hemocytes from Litopenaeus vannamei which were injected by WSSV (white spot syndrome virus) or PBS for 6 h using Illumina Hiseq 4000 platform. As a result, 3444 differentially expressed genes (DEGs), including 3240 up-regulated genes and 204 down-regulated genes, were identified from hemocytes after WSSV infection. Among these genes, 349 DEGs were correlated with innate immunity and categorized into seven groups based on their predictive function. Interestingly, 18 genes encoded putative neuropeptide precursors were induced significantly by WSSV infection. Furthermore, some genes were mapped to several typical processes in the NEI system, including proteolytic processing of prohormones, amino acid neurotransmitter pathways, biogenic amine biosynthesis and acetylcholine signaling pathway. Conclusions: The data suggested that WSSV infection triggers the activation of NEI in shrimp, which throws a light on the pivotal roles of NEI system mediated by hemocytes in shrimp antiviral immunity.
资助项目	Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48 (CARS-48)
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000463242500003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/154938]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fuxuan,Li, Shihao,Xiang, Jianhai,et al. Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection[J]. BMC GENOMICS,2019,20:14.
APA	Wang, Fuxuan,Li, Shihao,Xiang, Jianhai,&Li, Fuhua.(2019).Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection.BMC GENOMICS,20,14.
MLA	Wang, Fuxuan,et al."Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection".BMC GENOMICS 20(2019):14.