How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest

doi:10.1016/j.ejsobi.2018.11.004

	How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest
	Yang, Yang 1,2; Zhang, Xinyu 1,2; Wang, Huimin 1,2; Fu, Xiaoli 1,2; Wen, Xuefa 1,2; Zhang, Chuang 3; Chen, Fusheng 4; Wan, Songze 4
刊名	EUROPEAN JOURNAL OF SOIL BIOLOGY
	2019
卷号	90 页码:15-21
关键词	Understory vegetation Hydrolase activity Substrate affinity Catalytic efficiency Phospholipid fatty acids
ISSN号	1164-5563
DOI	10.1016/j.ejsobi.2018.11.004
通讯作者	Zhang, Xinyu(zhangxy@igsnrr.ac.cn)
英文摘要	To study how the understory vegetation influences the catalytic properties of extracellular hydrolases, we established a paired treatment experiment, with understory vegetation and litter removed from one treatment (understory removal, UR) and litter removed and the understory vegetation left intact in the other (control, CK), in a subtropical Chinese fir (Cunninghamia lanceolata) plantation. We used fluorescent substrates to determine the maximum velocity (V-max), the affinity of hydrolases to the substrates (K-m), and the catalytic efficiency (V-max/K-m) of three extracellular hydrolases (beta-1,4-glucosidase (beta G), beta-1,4-N-acetylglucosarninidase (NAG) and acid phosphatase (AP)). We found that the V-max, values for AG and NAG were 23.0% and 16.8% lower, respectively, in the UR treatment than in the CK treatment, and that the V-max for AP was similar in both treatments. The K-m and V-max/K-m for all the three hydrolases remained steady after the understory vegetation was removed. The soil C and N contents and the bacterial and fungal biomass were generally positively correlated with the V-max values. Thus, understory vegetation had more influence on the activities (V-max) than on the substrate affinities of extracellular hydrolases (K-m). Microbes tended to concentrate on maintaining the catalytic efficiency when the SOC contents were between 15.8 and 20.3 g kg(-1) when the understory vegetation was removed from a subtropical Chinese fir forest, and the catalytic efficiency could decrease under extremely low soil C contents. We suggest that understory vegetation should be maintained to sustain the potential microbial activity in subtropical Chinese fir forests.
资助项目	National Natural Science Foundation of China[41571251] ; National Natural Science Foundation of China[41571130043]
WOS关键词	MICROBIAL COMMUNITY COMPOSITION ; GROUND CARBON ALLOCATION ; ENZYME-ACTIVITIES ; KINETICS ; REMOVAL ; TEMPERATURE ; PLANTATION ; FERTILIZATION ; AVAILABILITY ; EFFICIENCY
WOS研究方向	Environmental Sciences & Ecology ; Agriculture
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000455693400003
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/50503]
专题	中国科学院地理科学与资源研究所
通讯作者	Zhang, Xinyu
作者单位	1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Key Lab Agr Water Resources, Shijiazhuang 050021, Hebei, Peoples R China 4.Jiangxi Agr Univ, Coll Forestry, Nanchang 330045, Jiangxi, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yang,Zhang, Xinyu,Wang, Huimin,et al. How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest[J]. EUROPEAN JOURNAL OF SOIL BIOLOGY,2019,90:15-21.
APA	Yang, Yang.,Zhang, Xinyu.,Wang, Huimin.,Fu, Xiaoli.,Wen, Xuefa.,...&Wan, Songze.(2019).How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest.EUROPEAN JOURNAL OF SOIL BIOLOGY,90,15-21.
MLA	Yang, Yang,et al."How understory vegetation affects the catalytic properties of soil extracellular hydrolases in a Chinese fir (Cunninghamia lanceolata) forest".EUROPEAN JOURNAL OF SOIL BIOLOGY 90(2019):15-21.