MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN

	MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN
	Fayyaz, Amna 1; Tufail, Muhammad Rizwan 1; Sahi, Shahbaz Talib 1; Masood, Atifa 2; Tazeen, Syeda Khola 2; Naseer, Ignaa 3; Farooq, Taimoor Hassan 4; Amrao, Luqman 1
刊名	FRESENIUS ENVIRONMENTAL BULLETIN
	2019
卷号	28 期号:3 页码:1770-1774
关键词	Chili Leaf Curl Disease Begomoviruses Chili Leaf Curl Virus Betasatellite RFLP
ISSN号	1018-4619
通讯作者	Masood, Atifa(dratifabot@yahoo.com)
英文摘要	Among many other plant diseases Chili Leaf Curl Disease (ChLCD) caused by Begomovirus complex is a crucial threat for chili worldwide. Chili Leaf Curl Virus (ChLCV) is monopartites DNA Begomovirus having DNA-A which comprises of 2754 nucleotides having six ORFs and betasetallite of size 1.3kb. Genomic DNA of infected chili leaf samples was extracted by CTAB method, collected from different field areas of Faisalabad. Circular molecules from genomic DNA were amplified by using Rolling Circle Amplification (RCA). Three samples showed successful RCA which further used in PCR amplification. For amplification of full length Begomovirus from RCA product universal primers were used. Betasatellite component associated with ChLCD was amplified with the help of PCR by using full-length universal primers 0301 and 002). RFLP analysis showed that single type of Begomovirus and diverse type of betasatellite molecules observed in infected chili samples.
资助项目	Higher Education Commission, Pakistan
WOS关键词	1ST REPORT ; GENETIC DIVERSITY ; DNA-BETA ; BEGOMOVIRUS ; DISEASE ; TOMATO ; PEPPER ; VECTOR
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PARLAR SCIENTIFIC PUBLICATIONS (P S P)
WOS记录号	WOS:000463297500015
资助机构	Higher Education Commission, Pakistan
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/48201]
专题	中国科学院地理科学与资源研究所
通讯作者	Masood, Atifa
作者单位	1.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan 2.Univ Lahore, Dept Bot, Sargodha, Punjab, Pakistan 3.Univ Chinese Acad Sci, Inst Geog Sci & Nat Resources, Key Lab Ecosyst Res Network Observat & Modeling, Beijing, Peoples R China 4.Fujian Agr & Forestry Univ, Forestry & Environm Res Inst, Fuzhou, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Fayyaz, Amna,Tufail, Muhammad Rizwan,Sahi, Shahbaz Talib,et al. MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2019,28(3):1770-1774.
APA	Fayyaz, Amna.,Tufail, Muhammad Rizwan.,Sahi, Shahbaz Talib.,Masood, Atifa.,Tazeen, Syeda Khola.,...&Amrao, Luqman.(2019).MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN.FRESENIUS ENVIRONMENTAL BULLETIN,28(3),1770-1774.
MLA	Fayyaz, Amna,et al."MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN".FRESENIUS ENVIRONMENTAL BULLETIN 28.3(2019):1770-1774.