a functional snP upstream of the ADRB2 gene is associated with COPD | |
li Zhong1,2,4; lu-Ming Dai5; Chang sun1,2; Xiao- hua Zhang1,3; Ya-Ping Zhang1,6; li Yu1,3; Jin-Xiu li1,3 | |
刊名 | International Journal of COPD
![]() |
2018 | |
期号 | 13页码:917–925 |
关键词 | β 2 -adrenergic Receptor Adrb2 Fev 1 Lung Polymorphism |
DOI | 10.2147/COPD.S151153 |
英文摘要 | Background: Previous studies have suggested that β 2 -adrenergic receptor (ADRB2) is associatedwith COPD. However, the role of genetic polymorphisms in ADRB2 on COPD has not beenevaluated yet. Methods: In this study, SNaPshot genotyping, luciferase assay, chromatin immunoprecipitationand real-time polymerase chain reaction were adopted to investigate the association betweenADRB2 genetic polymorphisms and COPD, comprehensively. Results: One single nucleotide polymorphism (rs12654778), located upstream of ADRB2,showed a significant association with COPD by the logistic regression analysis after adjusting forage, sex and smoking history (p=0.04) in 200 COPD patients and 222 controls from southwestChinese population. Furthermore, the luciferase assay indicated that rs12654778-A allele reducedthe relative promoter activity by ~26% compared with rs12654778-G allele (p=0.0034). Thechromatin immunoprecipitation analysis demonstrated that rs12654778 modulated the bindingaffinity of transcription factor neurofibromin 1. In addition, a significantly reduced expressionof ADRB2 in COPD patients was observed, compared with normal controls (p=0.017). Conclusion: Our findings suggest a previously unknown mechanism linking allele-specificeffects of rs12654778 on ADRB2 expression to COPD onset, for the first time. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.26:8080/handle/152453/12287] ![]() |
专题 | 昆明动物研究所_分子进化基因组学 |
通讯作者 | li Zhong |
作者单位 | 1.state Key laboratory for Conservation and Utilization of Bio- resource in Yunnan 2.College of life sciences 3.Key laboratory for animal genetic Diversity and evolution of high education in Yunnan Province, school of life sciences, Yunnan University 4.Provincial Demonstration Center for experimental Biology education, shaanxi normal University, Xi’an 5.Department of respiratory Critical Care Medicine 6.state Key laboratory of genetic resources and evolution, and Yunnan laboratory of Molecular Biology of Domestic animals, Kunming Institute of Zoology, Chinese academy of sciences, Kunming, China 7.Department of Thoracic surgery, The First affiliated hospital of Kunming Medical University, Kunming |
推荐引用方式 GB/T 7714 | li Zhong,lu-Ming Dai,Chang sun,et al. a functional snP upstream of the ADRB2 gene is associated with COPD[J]. International Journal of COPD,2018(13):917–925. |
APA | li Zhong.,lu-Ming Dai.,Chang sun.,Xiao- hua Zhang.,Ya-Ping Zhang.,...&Jin-Xiu li.(2018).a functional snP upstream of the ADRB2 gene is associated with COPD.International Journal of COPD(13),917–925. |
MLA | li Zhong,et al."a functional snP upstream of the ADRB2 gene is associated with COPD".International Journal of COPD .13(2018):917–925. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论