Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method

doi:10.1016/j.aca.2019.04.025

	Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method
	Qin, Hongqiang 1; Chen, Yao 1,3; Mao, Jiawei 1,3; Cheng, Kai 1; Sun, Deguang 2; Dong, Mingming 1; Wang, Lu 1; Wang, Liming 2; Ye, Mingliang 1
刊名	ANALYTICA CHIMICA ACTA
	2019-09-06
卷号	1070 页码:60-68
关键词	Glycoproteomics Site-specific glycoforms Glycosites Mass spectrometry Micro-heterogeneity of glycosylation
ISSN号	0003-2670
DOI	10.1016/j.aca.2019.04.025
通讯作者	Ye, Mingliang(mingliang@dicp.ac.cn)
英文摘要	Determination of site-specific glycoforms is the key to reveal the micro-heterogeneity of protein glycosylation at proteome level. Herein, we presented an integrated virtual multistage MS strategy to identify intact glycopeptides, which allowed the determination of site-specific glycoforms. In this strategy, the enzymatically de-glycosylated peptides and intact glycopeptides were mixed and analyzed in the same LC-MS/MS run. The acquired MS2 spectra of intact glycopeptides allowed determination of the glycans, and the MS2 spectra of the de-glycosylated peptides enabled the identification of peptide backbone sequences. Compared with the conventional multistage strategy, the peptide backbones could be directly identified by the MS2 of the de-glycopeptides with higher sensitivity. This strategy was first validated by analyzing the glycosites and site-specific glycoforms of mouse liver tissues. Then, it was applied to differential analysis of the glycoproteomes of hepatocellular carcinoma (HCC) and adjacent liver tissues. Compared with the identification scheme using only MS2 spectra of intact glycopeptides or glycosites, this approach enabled quantitative analysis on two levels, i.e. glycosites and site-specific glycoforms, simultaneously. Thus, it could be a powerful tool to characterize the subtle differences in the macro- and micro-heterogeneity of protein glycosylation for different samples. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	China State Key Basic Research Program Grants[2018YFC0910302] ; China State Key Basic Research Program Grants[2016YFA0501402] ; National Natural Science Foundation of China[21405516] ; National Natural Science Foundation of China[21775146] ; National Natural Science Foundation of China[81600046] ; National Natural Science Foundation of China[81430072] ; innovation program of science and research from the DICP, CAS[DICP TMSR201601] ; National Science Fund of China for Distinguished Young Scholars[21525524]
WOS关键词	SOLID-PHASE EXTRACTION ; INTACT N-GLYCOPEPTIDES ; TARGETED GLYCOPROTEOMICS ; MATCHING ALGORITHM ; GLYCAN STRUCTURE ; GLYCOSYLATION ; CANCER ; IDENTIFICATION ; PLATFORM ; ANTIGEN
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000467910000005
资助机构	China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; innovation program of science and research from the DICP, CAS ; innovation program of science and research from the DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; innovation program of science and research from the DICP, CAS ; innovation program of science and research from the DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; innovation program of science and research from the DICP, CAS ; innovation program of science and research from the DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; innovation program of science and research from the DICP, CAS ; innovation program of science and research from the DICP, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171875]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Ye, Mingliang
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qin, Hongqiang,Chen, Yao,Mao, Jiawei,et al. Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method[J]. ANALYTICA CHIMICA ACTA,2019,1070:60-68.
APA	Qin, Hongqiang.,Chen, Yao.,Mao, Jiawei.,Cheng, Kai.,Sun, Deguang.,...&Ye, Mingliang.(2019).Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method.ANALYTICA CHIMICA ACTA,1070,60-68.
MLA	Qin, Hongqiang,et al."Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method".ANALYTICA CHIMICA ACTA 1070(2019):60-68.