Quantitative analysis of protein orientation in membrane environments by kinase activity

doi:10.1016/j.jbiosc.2015.06.002

	Quantitative analysis of protein orientation in membrane environments by kinase activity
	Liu, Baoquan 1,2,3; Fan, Shengdi 1,2,3; Xiong, Wen 1,2,3; Quan, Chunshan 1,2,3; Zhang, Xuning 4; Wang, Lina 5; Jin, Liming 1,2,3
刊名	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
	2016-02-01
卷号	121 期号:2 页码:242-246
关键词	Agrc Protein Orientation Liposome Thiol-reactive Reagent Kinase Activity
ISSN号	1389-1723
DOI	10.1016/j.jbiosc.2015.06.002
文献子类	Article
英文摘要	AgrC is an integral membrane receptor protein with histidine kinase activity in the accessory gene regulator (agr) quorum-sensing system of Staphylococcus aureus. In this study, proteoliposomes were used as a model to investigate AgrC orientation. Many approaches have been described to determine membrane protein orientation, but they are often complicated and time consuming. In this study, AgrC orientation in liposomes was determined by thiol-reactive reagent labeling and a kinase activity assay. Our results suggest use of a kinase activity assay could get an accurate percentage of functional protein orientation and only cost nearly one-sixth of the time compared with the method based on thiol-reactive reagent labeling. We present an effective and rapid method for determining the orientation of membrane protein kinases like AgrC. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
WOS关键词	STAPHYLOCOCCUS-AUREUS ; RECONSTITUTION ; SOLUBILIZATION ; LIPOSOMES ; AGRC ; INFECTIONS ; DETERGENTS ; VIRULENCE ; MIXTURES ; RECEPTOR
WOS研究方向	Biotechnology & Applied Microbiology ; Food Science & Technology
语种	英语
出版者	SOC BIOSCIENCE BIOENGINEERING JAPAN
WOS记录号	WOS:000370887300018
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171266]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Quan, Chunshan
作者单位	1.Dalian Nationalities Univ, Econ & Technol Dev Zone, Minist Educ, Dalian 116600, Peoples R China 2.Dalian Nationalities Univ, Dept Life Sci, Econ & Technol Dev Zone, Dalian 116600, Peoples R China 3.Dalian Nationalities Univ, Key Lab Biotechnol & Resource Utilizat, State Ethn Affairs Commiss, Econ & Technol Dev Zone, Dalian 116600, Peoples R China 4.Dalian Polytechn Univ, Sch Biol Engn, 1 Qinggongyuan, Dalian 116034, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhong Shan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liu, Baoquan,Fan, Shengdi,Xiong, Wen,et al. Quantitative analysis of protein orientation in membrane environments by kinase activity[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(2):242-246.
APA	Liu, Baoquan.,Fan, Shengdi.,Xiong, Wen.,Quan, Chunshan.,Zhang, Xuning.,...&Jin, Liming.(2016).Quantitative analysis of protein orientation in membrane environments by kinase activity.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(2),242-246.
MLA	Liu, Baoquan,et al."Quantitative analysis of protein orientation in membrane environments by kinase activity".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.2(2016):242-246.