Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification

doi:10.1016/j.talanta.2016.01.016

	Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification
	Pei, Xiaojing; Huang, Hongduan; Chen, Yang; Li, Chenxi; Liu, Feng; Li, Na
刊名	TALANTA
	2016-07-01
卷号	154 页码:567-573
关键词	Fluorescence Anisotropy Double-stranded Dna Leveling-off Phenomenon Middle-site-labeling c Overhang Mutiple Number Of Labels
ISSN号	0039-9140
DOI	10.1016/j.talanta.2016.01.016
文献子类	Article
英文摘要	Fluorescence anisotropy, known as a simple, homogeneous and cost-effective analytical technology, is an invaluable technique for studying the micro -environmental changes of the dye associated with the molecular interactions. An in-depth understanding of the variables affecting the fluorescence anisotropy signal can facilitate better experimental designs to effectively improve the analytical performance. This work is a follow-up effort in evaluating the factors that can significantly influence fluorescence anisotropy. We systematically studied fluorescence anisotropy of dsDNA with the changing length based on dye-DNA interactions, with the fluorophores in the end -labeling, the middle -site -labeling, and multiple number of labeling manners. The fluorescence anisotropy value and the base -pair response dynamic range could be expanded by labeling the fluorophores in the middle of dsDNA and increasing the number of labels on dsDNA. The C overhang configuration in the end -labeling manner could enhance the fluorescence anisotropy signal but not expand the base -pair response range. Results from all the labeling fluorophores reinforced the leveling -off effect, i.e., the fluorescence anisotropy signal does not response to the increased length of the DNA duplex when the length is larger than a critical number of base pairs. These findings provide perspectives about choosing appropriate fluorescent dyes and labeling sites for simple and universal fluorescence anisotropy designs in various applications. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	SYBR-GREEN-I ; DOUBLE-STRANDED DNA ; SMALL MOLECULES ; SPECTROSCOPIC CHARACTERIZATION ; SENSITIVE DETECTION ; THIAZOLE ORANGE ; GROOVE-BINDING ; APTAMER ; POLARIZATION ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000376696400076
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170982]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Na
作者单位	Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, BNLMS,Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Pei, Xiaojing,Huang, Hongduan,Chen, Yang,et al. Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification[J]. TALANTA,2016,154:567-573.
APA	Pei, Xiaojing,Huang, Hongduan,Chen, Yang,Li, Chenxi,Liu, Feng,&Li, Na.(2016).Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification.TALANTA,154,567-573.
MLA	Pei, Xiaojing,et al."Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification".TALANTA 154(2016):567-573.