Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds

doi:10.1016/j.chroma.2016.04.031

	Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds
	Liu, Dan 1; Hong, Zhilai 1; Gao, Mingzhe 1; Wang, Zhixin 1; Gu, Ming 2; Zhang, Xiaozhe 1; Xiao, Hongbin 1,3
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016-05-27
卷号	1448 页码:49-57
关键词	Hydrophilic Organic/salt-containing Aqueous Two-phase System Counter-current Chromatography Hydrophilic Compounds Polarity Selectivity
ISSN号	0021-9673
DOI	10.1016/j.chroma.2016.04.031
文献子类	Article
英文摘要	The essential attributes of a solvent system for separation polar compounds on CCC are polarity, selectively and performance. Here, hydrophilic organic/salt-containing aqueous two-phase system (HO/S TPS) was evaluated as an alternative solvent system for CCC separation of polar compounds. Polarity measurements based on Rohrschneider-Snyder parameter was developed as quantitative assessing the polarity of HO/S TPS and comparing with an organic/aqueous system. All investigated 1-butanol/ethanol/saturated ammonium sulfate solution/water (BEAsWat) and 1-butanol/ethanol/saturated dipotassium hydrogen phosphate solution/water (BEDhpWat) systems with polarity values of organic phase from 4.5 to 6.8, were more polar than chloroform/methanol/water (1/1/1). The considerable water content of BEAsWat and BEDhpWat (0/1/1/1/) was 45.4 and 42.6% (w%) of hydrophilic organic phase, and 66.4 and 51.2% (w%) of salt-containing aqueous phase, respectively, closed to conventional aqueous two-phase system. Therefore, the polarity of HO/S TPS is in the middle of organic/aqueous and aqueous two-phase system. The LogK(C) values of twenty four polar compounds as model mixture confirmed that the polarities of HO/S TPSs were matched to that of the polar compounds and shown to be a very selective technique capable of separating positional isomers. Moreover, BEAsWat and BEDhpWat systems can be easily retained in CCC column with suitable elution mode. The hydrodynamic behavior reversion of HO/S TPS on hydrodynamic CCC was observed and was tentatively explained based on the density difference. Finally, caffeoylquinic acid isomers and dihydroxybenzoic acid isomers were successfully separated with HO/S TPS on CCC, respectively. Those results demonstrate that HO/S TPS on CCC is a performant and stable way to separate polar compounds from natural products. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	FERMENTATION BROTHS ; SOLVENT SYSTEMS ; SALVIA-MILTIORRHIZA ; ACID-B ; SEPARATION ; PURIFICATION ; EXTRACTION ; 1,3-PROPANEDIOL ; 2,3-BUTANEDIOL ; PARTITION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000376211600006
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170958]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Xiaozhe; Xiao, Hongbin
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Beijing Univ Chinese Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dan,Hong, Zhilai,Gao, Mingzhe,et al. Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1448:49-57.
APA	Liu, Dan.,Hong, Zhilai.,Gao, Mingzhe.,Wang, Zhixin.,Gu, Ming.,...&Xiao, Hongbin.(2016).Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds.JOURNAL OF CHROMATOGRAPHY A,1448,49-57.
MLA	Liu, Dan,et al."Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds".JOURNAL OF CHROMATOGRAPHY A 1448(2016):49-57.