Ultra-high performance liquid chromatography with linear ion trap-Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts

doi:10.1002/jssc.201500981

	Ultra-high performance liquid chromatography with linear ion trap-Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts
	Zhang, Xiaozhe 1; Wang, Zhixin 1; Qu, Yang 1; Wang, Li 1; Xiao, Hongbin 2
刊名	JOURNAL OF SEPARATION SCIENCE
	2016-04-01
卷号	39 期号:7 页码:1379-1388
关键词	Fragment Ions Linear Ion Trap-orbitrap Stellera Chamaejasme Traditional Chinese Medicine Ultra-high Performance Liquid Chromatography
ISSN号	1615-9306
DOI	10.1002/jssc.201500981
文献子类	Article
英文摘要	Stellera chamaejasme, a famous toxic herb, has been used in traditional Chinese medicine to treat various diseases. For decades, increasing attention inmodern pharmacological studies has been drawn to S. chamaejasme because of its potential anti-tumor, anti-virus, and anti-fungus activities. However, due to the intrinsic complexity of chemical constitutes, hardly any investigations formed an overall recognition for the chemical profiles of this herb. In this study, a rapid and sensitive ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry method was developed to characterize the chemical components of S. chamaejasme extracts. Based on optimized ultra-high performance liquid chromatography and mass spectrometry conditions and systematic fragment ions-based strategy, a total of 47 components including flavones, diterpenes, coumarins, and lignans were simultaneously detected and identified or tentatively identified for the first time. The MSn fragmentation patterns of all the characterized compounds in positive or negative electrospray ionization modes were also explored and summarized. These results provided essential data for further pharmacological research on S. chamaejasme. Moreover, the method was demonstrated to be an efficient tool for rapid qualitative analysis of secondary metabolites from natural resources.
WOS关键词	DAPHNANE-TYPE DITERPENOIDS ; WIKSTROEMIA-RETUSA ; ROOTS ; IDENTIFICATION ; CONSTITUENTS ; L. ; COMPOUND
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000374029500022
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170726]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Xiaozhe
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Beijing Univ Chinese Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaozhe,Wang, Zhixin,Qu, Yang,et al. Ultra-high performance liquid chromatography with linear ion trap-Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(7):1379-1388.
APA	Zhang, Xiaozhe,Wang, Zhixin,Qu, Yang,Wang, Li,&Xiao, Hongbin.(2016).Ultra-high performance liquid chromatography with linear ion trap-Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts.JOURNAL OF SEPARATION SCIENCE,39(7),1379-1388.
MLA	Zhang, Xiaozhe,et al."Ultra-high performance liquid chromatography with linear ion trap-Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts".JOURNAL OF SEPARATION SCIENCE 39.7(2016):1379-1388.