The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer

doi:10.1007/s00216-016-9797-0

	The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer
	Xu, Chuanliang 3; Zhou, Yang 1,2; Song, Ruixiang 3; Zhang, Zhensheng 3; Lu, Xin 1; Zeng, Zhongda 1; Hu, Chunxiu 1; Liu, Xinyu 1,2; Li, Yanli 1; Hou, Jianguo 3
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2016-09-01
卷号	408 期号:24 页码:6741-6749
关键词	Metabolomics Bladder Cancer Metabolic Profiling Gas Chromatography-mass Spectrometry Pseudotargeted Plasma
ISSN号	1618-2642
DOI	10.1007/s00216-016-9797-0
文献子类	Article
英文摘要	Bladder cancer (BC) is a fatal malignancy with considerable mortality. BC urinary metabolomics has been extensively investigated for biomarker discovery, but few BC blood metabolomic studies have been performed. Hence, a plasma pseudotargeted metabolomic method based on gas chromatography-mass spectrometry with selected ion monitoring (GC-MS-SIM) was developed to study metabolic alterations in BC. The analytical performance of the developed method was compared with that of a nontargeted method. The relative standard deviation (RSD) values of 89 and 70.7 % of the peaks obtained using the pseudotargeted and nontargeted methods, respectively, were less than 20 %. The Pearson correlations of 90.7 and 78.3 % of the peaks obtained using the pseudotargeted and nontargeted methods, respectively, exceeded 0.90 in the linearity evaluation. Compared with the nontargeted method, the signal-to-noise ratios (S/N) of 97.9 and 69.3 % of the peaks increased two- and fivefold, respectively. The developed method was fully validated, with good precision, recovery, and stability of the trimethylsilyl (TMS) derivatives. The method was applied to investigate BC. Significant increases in the contents of metabolites involved in, for example, the pentose phosphate pathway (PPP) and nucleotide and fatty acid synthesis were found in the high-grade (HG) BC group compared to the healthy control (HC) group. These differences imply that the activated PPP may regulate BC cell proliferation by promoting lipid and nucleotide biosynthesis and the detoxification of reactive oxygen species (ROS). These results illustrate that the plasma pseudotargeted method is a powerful tool for metabolic profiling.
WOS关键词	GAS CHROMATOGRAPHY/MASS SPECTROMETRY ; PENTOSE-PHOSPHATE PATHWAY ; MASS SPECTROMETRY ; BIOMARKER DISCOVERY ; CROSS-VALIDATION ; TOBACCO-LEAVES ; EXPRESSION ; URINARY ; CLASSIFICATION ; PROGRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000382883300021
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170256]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xu, Chuanliang; Lu, Xin
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Secondary Mil Med Univ, Shanghai Changhai Hosp, Dept Urol, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chuanliang,Zhou, Yang,Song, Ruixiang,et al. The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(24):6741-6749.
APA	Xu, Chuanliang.,Zhou, Yang.,Song, Ruixiang.,Zhang, Zhensheng.,Lu, Xin.,...&Xu, Guowang.(2016).The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(24),6741-6749.
MLA	Xu, Chuanliang,et al."The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.24(2016):6741-6749.