Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation

doi:10.18632/oncotarget.9479

	Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation
	He, Qianqian 1,2; Zhang, Lei 1,2; Wang, Jihao 1,2,3; Wang, HongLei 4; Wang, Wenchao 1,2; Li, Zhiyuan 1,2; Liu, Juanjuan 1,2; Yang, Xingxing 1,2; Ji, Xinmiao 1,2; Luo, Yan 1,2
刊名	ONCOTARGET
	2016-07-05
卷号	7 期号:27 页码:41527-41539
关键词	Egfr Magnetic Field Stm Cancer
ISSN号	1949-2553
DOI	10.18632/oncotarget.9479
文献子类	Article
英文摘要	Static magnetic fields (SMFs) can affect cell proliferation in a cell-type and intensity-dependent way but the mechanism remains unclear. At the same time, although the diamagnetic anisotropy of proteins has been proposed decades ago, the behavior of isolated proteins in magnetic fields has not been directly observed. Here we show that SMFs can affect isolated proteins at the single molecular level in an intensity-dependent manner. We found that Epidermal Growth Factor Receptor (EGFR), a protein that is overexpressed and highly activated in multiple cancers, can be directly inhibited by SMFs. Using Liquid-phase Scanning Tunneling Microscopy (STM) to examine pure EGFR kinase domain proteins at the single molecule level in solution, we observed orientation changes of these proteins in response to SMFs. This may interrupt inter-molecular interactions between EGFR monomers, which are critical for their activation. In molecular dynamics (MD) simulations, 1-9T SMFs caused increased probability of EGFR in parallel with the magnetic field direction in an intensity-dependent manner. A superconducting ultrastrong 9T magnet reduced proliferation of CHO-EGFR cells (Chinese Hamster Ovary cells with EGFR overexpression) and EGFR-expressing cancer cell lines by similar to 35%, but minimally affected CHO cells. We predict that similar effects of magnetic fields can also be applied to some other proteins such as ion channels. Our paper will help clarify some dilemmas in this field and encourage further investigations in order to achieve a better understanding of the biological effects of SMFs.
WOS关键词	MOLECULAR-DYNAMICS SIMULATION ; GROWTH-FACTOR RECEPTOR ; IN-VIVO ; DIAMAGNETIC ANISOTROPY ; ANTITUMOR EFFICACY ; PLASMA-MEMBRANE ; EXPOSURE ; ACTIVATION ; ALIGNMENT ; PROTEINS
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000380942600046
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170242]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Guohui; Lu, Qingyou; Zhang, Xin
作者单位	1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Liaoning, Peoples R China 5.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Nanjing 210093, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	He, Qianqian,Zhang, Lei,Wang, Jihao,et al. Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation[J]. ONCOTARGET,2016,7(27):41527-41539.
APA	He, Qianqian.,Zhang, Lei.,Wang, Jihao.,Wang, HongLei.,Wang, Wenchao.,...&Zhang, Xin.(2016).Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation.ONCOTARGET,7(27),41527-41539.
MLA	He, Qianqian,et al."Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation".ONCOTARGET 7.27(2016):41527-41539.