Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis

doi:10.1002/jcla.21943

	Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis
	Fang, Yanhua 6,7; Gao, Peng 1,3,5,6,7; Sun Hongzhi 5,6,7; Hu, Zhansheng 5; Zhu, Zhitu 5; Cao, Yunfeng 1,5,6,7; Wang, Lixuan 6,7; Sun, Xiaoyu 6,7; Dong, Jun 6,7; Fang, Zhongze 2,4,6,7
刊名	JOURNAL OF CLINICAL LABORATORY ANALYSIS
	2016-11-01
卷号	30 期号:6 页码:823-830
关键词	Stroke Mass Spectrometry Metabolomics
ISSN号	0887-8013
DOI	10.1002/jcla.21943
文献子类	Article
英文摘要	Cerebral infarction (CI) and intracerebral hemorrhage are lethal cerebrovascular diseases, sometimes sharing similar clinical manifestations but with distinct therapeutic strategies. Delayed treatment usually resulted in poor prognosis. A timely diagnosis report is highly warranted especially in emergency. One hundred twenty-nine CI patients, 73 intracerebral hemorrhage (ICH) patients, and 98 controls were enrolled in this study. A direct injection mass spectrometry metabolomics approach was adopted using dried blood spot samples. This targeted metabolomics analysis focused on absolute quantitation of 23 amino acids, 26 carnitine/carnitine esters, and 22 calculated ratios parameters. Compared to the normal control group, CI and ICH showed distinct metabolite changes, respectively. For stroke differentiation, Tyr, C5-OH/C0, Cit, Asn, Pro, Val, Arg/Orn, Leu, and Val/Phe were elevated in the CI group. On the contrary, C5:1, Phe/Tyr, (C0 + C2 + C3 + C16 + C18:1)/Cit, and Met/Leu were of lower levels in the CI group. Using regression model based on some of the above-mentioned parameters, 79.07% of stroke patients from a new set could be definitely confirmed. This study proved the targeted metabolomics analysis was a promising tool for rapid and timely stroke differentiation.
WOS关键词	REGIONAL BURDEN ; GLOBAL BURDEN ; BRAIN-DAMAGE ; LC-MS/MS ; DISEASE ; METABONOMICS
WOS研究方向	Medical Laboratory Technology
语种	英语
出版者	JOHN WILEY & SONS INC
WOS记录号	WOS:000386935200005
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169902]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Sun Hongzhi
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin, Peoples R China 3.Dalian Sixth Peoples Hosp, Clin Lab, Dalian, PR, Peoples R China 4.Tianjin Med Univ, Dept Microbiol, Sch Basic Med Sci, Tianjin, Peoples R China 5.Liaoning Med Univ, Affiliated Hosp 1, Jinzhou 121000, Peoples R China 6.Chinese Acad Sci, Joint Ctr Translat Med, Dalian Inst Chem Phys, Dalian, Peoples R China 7.Liaoning Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Fang, Yanhua,Gao, Peng,Sun Hongzhi,et al. Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2016,30(6):823-830.
APA	Fang, Yanhua.,Gao, Peng.,Sun Hongzhi.,Hu, Zhansheng.,Zhu, Zhitu.,...&Xu, Xiaoxue.(2016).Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis.JOURNAL OF CLINICAL LABORATORY ANALYSIS,30(6),823-830.
MLA	Fang, Yanhua,et al."Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis".JOURNAL OF CLINICAL LABORATORY ANALYSIS 30.6(2016):823-830.