Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach

doi:10.1002/pmic.201600183

	Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach
	Gao, Huiyuan 1; Ye, Xueting 1; Zhao, Nan 2; Yu, Xi 3; Han, Xiaoli 1; Zhang, Xiaozhe 2
刊名	PROTEOMICS
	2016-11-01
卷号	16 期号:21 页码:2788-2791
关键词	Ginseng Peptides Nano-lc-ms Ms Peaks Plant Proteomics Sequencing
ISSN号	1615-9853
DOI	10.1002/pmic.201600183
文献子类	Article
英文摘要	Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of precursor ions indicated that Ginseng is a peptide-rich herb in comparison of a number of commonly used herbs. The Ginseng peptides were then extracted and submitted to nano-LC-MS/MS analysis using different fragmentation modes, including CID, high-energy collisional dissociation, and electron transfer dissociation. Further database search and de novo sequencing allowed the identification of total 308 peptides, some of which might have important biological activities. This study illustrates the abundance and sequences of endogenous Ginseng peptides, thus providing the information of more candidates for the screening of active compounds for future biological research and drug discovery studies.
WOS关键词	CELLS ; ROOT
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000387168600007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169832]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Gao, Huiyuan; Zhang, Xiaozhe
作者单位	1.Shenyang Pharmaceut Univ, Key Lab Struct Based Drug Design & Discovery, Minist Educ, Wenhua Rd 103, Shenyang, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biol Technol, Dalian, Peoples R China 3.Natl Univ Singapore, Food Sci & Technol Programme, Dept Chem, Singapore, Singapore
推荐引用方式 GB/T 7714	Gao, Huiyuan,Ye, Xueting,Zhao, Nan,et al. Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach[J]. PROTEOMICS,2016,16(21):2788-2791.
APA	Gao, Huiyuan,Ye, Xueting,Zhao, Nan,Yu, Xi,Han, Xiaoli,&Zhang, Xiaozhe.(2016).Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach.PROTEOMICS,16(21),2788-2791.
MLA	Gao, Huiyuan,et al."Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach".PROTEOMICS 16.21(2016):2788-2791.