A lysosomal probe for monitoring of pH in living cells and ovarian tumour

doi:10.1016/j.dyepig.2016.12.043

	A lysosomal probe for monitoring of pH in living cells and ovarian tumour
	Li, Bo 1; Ge, Guang Bo 2; Peng, Xiaojun 1; Liu, Fengyu 1; Sun, Shiguo 3; Wen, Lanlan 1; Yuan, Ye 1; Zhang, Ran 1
刊名	DYES AND PIGMENTS
	2017-04-01
卷号	139 页码:318-325
关键词	Monitoring Ph Lysosome Fluorescent Probe Cells Ovarian Tumour
ISSN号	0143-7208
DOI	10.1016/j.dyepig.2016.12.043
文献子类	Article
英文摘要	Intracellular pH is important in regulating various cellular behaviour and pathological conditions. We synthesised a rhodamine-based lysosomal pH probe (hereafter referred to as RD) with a pKa of 4.10. The probe exhibited favourable "on-off' reversibility between pH 3.25 and 7.20 for at least five cycles. RD can be employed to distinguish cancer cells from normal cells on the basis of different fluorescence response. RD performed well in the detection of lysosomal pH induced by artesunate, chloroquine and dexamethasone in MCF-7 cells. The intense fluorescence of RD in ovarian tumour indicated the higher acidity in tumour tissues than that in normal tissues. Furthermore, RD worked well in the imaging of excised mouse stomach and living mice under acidic stimulation. These data demonstrate the applicability of RD in monitoring pH in complex biological systems.(C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词	RATIOMETRIC FLUORESCENT-PROBE ; IN-VIVO ; CANCER-CELLS ; INTRACELLULAR PH ; RHODAMINE-B ; MITOCHONDRIA ; SENSOR ; PROVIDES ; OXYGEN
WOS研究方向	Chemistry ; Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000394063800037
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169516]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Fengyu; Sun, Shiguo
作者单位	1.Dalian Univ Technol, Sch Chem, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Shihezi Univ, Sch Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832000, Peoples R China
推荐引用方式 GB/T 7714	Li, Bo,Ge, Guang Bo,Peng, Xiaojun,et al. A lysosomal probe for monitoring of pH in living cells and ovarian tumour[J]. DYES AND PIGMENTS,2017,139:318-325.
APA	Li, Bo.,Ge, Guang Bo.,Peng, Xiaojun.,Liu, Fengyu.,Sun, Shiguo.,...&Zhang, Ran.(2017).A lysosomal probe for monitoring of pH in living cells and ovarian tumour.DYES AND PIGMENTS,139,318-325.
MLA	Li, Bo,et al."A lysosomal probe for monitoring of pH in living cells and ovarian tumour".DYES AND PIGMENTS 139(2017):318-325.