2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol

doi:10.1016/j.bios.2018.02.021

	2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol
	Wu, Zhong-Shuai 3; Wang, Xiaohui 4; Chen, Jiping 2; Wu, Lingxia 1,2; Lu, Xianbo 2; Dhanjai 2; Zheng, Shuanghao 1,3; Dong, Yanfeng 3
刊名	BIOSENSORS & BIOELECTRONICS
	2018-06-01
卷号	107 页码:69-75
关键词	Mxene Transition Metal Carbides Biosensor Tyrosinase Phenol
ISSN号	0956-5663
DOI	10.1016/j.bios.2018.02.021
文献子类	Article
英文摘要	MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with-OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensidve and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5 mu mol L-1, with a low detection limit of 12 nmol L-1 and a sensitivity of 414.4 mA M-1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis.
WOS关键词	2-DIMENSIONAL TITANIUM CARBIDE ; WATER-SOLUBLE GRAPHENE ; TI3C2 MXENE ; FUNCTIONALIZED GRAPHENE ; DIRECT ELECTROCHEMISTRY ; AQUEOUS-MEDIA ; ION BATTERIES ; ADSORPTION ; INTERCALATION ; EXFOLIATION
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000428006900010
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169120]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Lu, Xianbo
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, 457 Zhongshan Rd, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Wu, Zhong-Shuai,Wang, Xiaohui,Chen, Jiping,et al. 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol[J]. BIOSENSORS & BIOELECTRONICS,2018,107:69-75.
APA	Wu, Zhong-Shuai.,Wang, Xiaohui.,Chen, Jiping.,Wu, Lingxia.,Lu, Xianbo.,...&Dong, Yanfeng.(2018).2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol.BIOSENSORS & BIOELECTRONICS,107,69-75.
MLA	Wu, Zhong-Shuai,et al."2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol".BIOSENSORS & BIOELECTRONICS 107(2018):69-75.